Overview

  • Product name
    Anti-Cdc37 antibody [EPR4934]
    See all Cdc37 primary antibodies
  • Description
    Rabbit monoclonal [EPR4934] to Cdc37
  • Tested applications
    Suitable for: WB, IP, IHC-P, Flow Cytmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Cdc37.

  • Positive control
    • Jurkat, A431, K562, LnCaP and SW480 cell lysates; Human breast ductal infiltrating carcinoma
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function
      Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity.
    • Sequence similarities
      Belongs to the CDC37 family.
    • Post-translational
      modifications
      Constitutively sumoylated by UBE2I.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • CDC 37 antibody
      • Cdc37 antibody
      • CDC37 cell division cycle 37 homolog antibody
      • CDC37 cell division cycle 37 S cerevisiae homolog antibody
      • CDC37 cell division cycle 37, S cerevisiae, homolog of antibody
      • Cdc37 homolog antibody
      • CDC37 protein antibody
      • CDC37_HUMAN antibody
      • CDC37A antibody
      • cell division cycle 37 antibody
      • Cell division cycle 37 homolog antibody
      • Hsp90 chaperone protein kinase targeting subunit antibody
      • Hsp90 chaperone protein kinase targeting subunit p50Cdc37 antibody
      • Hsp90 chaperone protein kinase-targeting subunit antibody
      • Hsp90 co chaperone Cdc37 antibody
      • Hsp90 co-chaperone Cdc37 antibody
      • p50 antibody
      • p50Cdc37 antibody
      • S cerevisiae hypothetical protein CDC37 antibody
      see all

    Images

    • All lanes : Anti-Cdc37 antibody [EPR4934] (ab109419) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : LnCaP cell lysate
      Lane 5 : SW480 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 44 kDa
    • ab109419, at 1/250 dilution, staining Cdc37 in Human breast ductal infiltrating carcinoma by Immunohistochemistry.

    References

    This product has been referenced in:
    • Synoradzki K & Bieganowski P Middle domain of human Hsp90 isoforms differentially binds Aha1 in human cells and alters Hsp90 activity in yeast. Biochim Biophys Acta 1853:445-52 (2015). Read more (PubMed: 25486457) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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