Overview

  • Product nameAnti-Cdc37 (phospho S13) antibody
    See all Cdc37 primary antibodies
  • Description
    Rabbit polyclonal to Cdc37 (phospho S13)
  • Specificityab61797 detects Cdc37 only when phosphorylated at serine 13.
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human Cdc37 around the phosphorylation site of serine 13 (E-V-SP-D-D).

  • Positive control
    • Human placenta tissue and NIH 3T3 cell extracts.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab61797 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).

Target

  • FunctionCo-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity.
  • Sequence similaritiesBelongs to the CDC37 family.
  • Post-translational
    modifications
    Constitutively sumoylated by UBE2I.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC 37 antibody
    • Cdc37 antibody
    • CDC37 cell division cycle 37 homolog antibody
    • CDC37 cell division cycle 37 S cerevisiae homolog antibody
    • CDC37 cell division cycle 37, S cerevisiae, homolog of antibody
    • Cdc37 homolog antibody
    • CDC37 protein antibody
    • CDC37_HUMAN antibody
    • CDC37A antibody
    • cell division cycle 37 antibody
    • Cell division cycle 37 homolog antibody
    • Hsp90 chaperone protein kinase targeting subunit antibody
    • Hsp90 chaperone protein kinase targeting subunit p50Cdc37 antibody
    • Hsp90 chaperone protein kinase-targeting subunit antibody
    • Hsp90 co chaperone Cdc37 antibody
    • Hsp90 co-chaperone Cdc37 antibody
    • p50 antibody
    • p50Cdc37 antibody
    • S cerevisiae hypothetical protein CDC37 antibody
    see all

Anti-Cdc37 (phospho S13) antibody images

  • All lanes : Anti-Cdc37 (phospho S13) antibody (ab61797) at 1/500 dilution

    Lane 1 : NIH 3T3 cell extracts
    Lane 2 : NIH 3T3 cell extracts with immunising peptide


    Predicted band size : 44 kDa
    Observed band size : 44 kDa
  • ab61797 at 1/50 - 1/100 dilution staining Cdc37 in human placenta by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

References for Anti-Cdc37 (phospho S13) antibody (ab61797)

This product has been referenced in:
  • Quotti Tubi L  et al. Inhibition of protein kinase CK2 with the clinical-grade small ATP-competitive compound CX-4945 or by RNA interference unveils its role in acute myeloid leukemia cell survival, p53-dependent apoptosis and daunorubicin-induced cytotoxicity. J Hematol Oncol 6:78 (2013). WB ; Human . Read more (PubMed: 24283803) »
  • Xavier CP  et al. Phosphorylation of CRN2 by CK2 regulates F-actin and Arp2/3 interaction and inhibits cell migration. Sci Rep 2:241 (2012). WB ; Mouse . Read more (PubMed: 22355754) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"