Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Cdc4/ Fbw7/ hSel 10.
(Peptide available as ab12311.)
Our Abpromise guarantee covers the use of ab12292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 45,110 kDa (predicted molecular weight: 80 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IP||Use a concentration of 5 µg/ml. PubMed: 20388706|
Western blot using ab12292 at 1/500.
Lane 1: HeLa nuclear
Lane 2: HeLa whole cell
Lane 3: A431 whole cell
Lane 4: Jurkat whole cell
Lane 5: HEK293 whole cell
Lane 6: HeLa nuclear + blocking peptide (ab12311)
Lane 7: HeLa whole cell + blocking peptide (ab12311)
Lane 8: A431 whole cell + blocking peptide (ab12311)
Lane 9: Jurkat whole cell + blocking peptide (ab12311)
Lane 10: HEK293 whole cell + blocking peptide (ab12311)
Secondary: Goat polyclonal to Rabbit IgG HRP ab6741 (1/5000)
Exposure time: 10 seconds.
20µg of cell lysate per lane
Blocking peptide used at 1µg/ml.
Expected band size at 80kDa.
Additional bands seen at 45, 55 and 150 kDa. We are uncertain as to the identity of these bands.