Overview

  • Product nameAnti-CDC42 antibody
    See all CDC42 primary antibodies
  • Description
    Rabbit polyclonal to CDC42
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
    Predicted to work with: Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide:

    DDPSTIEKLA KNKQKPIT

    , corresponding to internal sequence amino acids 121-138 of Human CDC42.

  • Positive control
    • Rat brain tissue lysate, MCF-7 cell lysate, HeLa cell lysate, SMMC whole cell lysate, U87 cell lysate, CDC42 recombinant protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab109553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use a concentration of 1 µg/ml.
ICC Use a concentration of 1 µg/ml.

Target

  • FunctionPlasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
  • Post-translational
    modifications
    AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • FormThere are 2 isoforms produced by alternative splicing. Isoform 1 also known as: Brain; Isoform 2 also known as: Placental.
  • Alternative names
    • CDC42 antibody
    • CDC42_HUMAN antibody
    • CDC42Hs antibody
    • Cell division control protein 42 homolog antibody
    • Cell division cycle 42 (GTP binding protein 25kDa) antibody
    • Cell division cycle 42 antibody
    • dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody
    • dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody
    • G25K antibody
    • G25K GTP-binding protein antibody
    • Growth regulating protein antibody
    • GTP binding protein 25kDa antibody
    • Small GTP binding protein CDC42 antibody
    • TKS antibody
    see all

Anti-CDC42 antibody images

  • All lanes : Anti-CDC42 antibody (ab109553) at 1 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : MCF-7 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : SMMC whole cell lysate
    Lane 5 : U87 whole cell lysate


    Predicted band size : 21 kDa
  • All lanes : Anti-CDC42 antibody (ab109553) at 1 µg/ml

    Lane 1 : CDC42 recombinant protein at 0.01 µg
    Lane 2 : CDC42 recombinant protein at 0.005 µg
    Lane 3 : CDC42 recombinant protein at 0.0025


    Predicted band size : 21 kDa

References for Anti-CDC42 antibody (ab109553)

This product has been referenced in:
  • Francis MK  et al. Endocytic membrane turnover at the leading edge is driven by a transient interaction between Cdc42 and GRAF1. J Cell Sci 128:4183-95 (2015). Read more (PubMed: 26446261) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"