Anti-CDC42 antibody (ab155940)
Key features and details
- Rabbit polyclonal to CDC42
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human, Non human primates, African green monkey
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CDC42 antibody
See all CDC42 primary antibodies -
Description
Rabbit polyclonal to CDC42 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Non human primates, African green monkey -
Immunogen
Truncated Human recombinant protein, corresponding to C-terminal amino acid residues in Human CDC42 (P60953).
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Positive control
- A431, C6, HepG2 and HeLa cells; Human pancreas tissue; HeLa, HepG2, 293T, A431, Jurkat, MCF7, U2-OS, K652, COS7, MEF and 3T3L1 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 30% Glycerol (glycerin, glycerine), 69% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000. Predicted molecular weight: 21 kDa.
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IHC-P |
1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF | (1) |
1/100.
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Notes |
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WB
1/1000. Predicted molecular weight: 21 kDa. |
IHC-P
1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100. |
Target
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Function
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia. -
Sequence similarities
Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily. -
Post-translational
modificationsAMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 998 Human
- Entrez Gene: 12540 Mouse
- Entrez Gene: 64465 Rat
- Omim: 116952 Human
- SwissProt: P60953 Human
- SwissProt: P60766 Mouse
- SwissProt: Q8CFN2 Rat
- Unigene: 467637 Human
see all -
Form
There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: Brain; Isoform 2 also known as: Placental. -
Alternative names
- CDC42 antibody
- CDC42_HUMAN antibody
- CDC42Hs antibody
see all
Images
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All lanes : Anti-CDC42 antibody (ab155940) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : 293T whole cell lysate
Lane 4 : A431 whole cell lysate
Lane 5 : Jurkat whole cell lysate
Lane 6 : MCF7 whole cell lysate
Lane 7 : U2-OS whole cell lysate
Lane 8 : K526 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 9 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 10 : MEF whole cell lysate
Lane 11 : 3T3L1 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 21 kDa
4-20% Tris HCl -
Immunofluorescent analysis of A431 cells, labeling CDC42 with ab155940 at 1/20 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is also shown.
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Immunofluorescent analysis of C6 cells, labeling CDC42 with ab155940 at 1/20 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is also shown.
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Immunofluorescent analysis of HepG2 cells, labeling CDC42 with ab155940 at 1/200 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is also shown.
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Immunofluorescent analysis of HeLa cells, labeling CDC42 with ab155940 at 1/100 dilution. Blue: Nuclei stained with Hoechst 33342.
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Immunohistochemical analysis of deparaffinised Human pancreas tissue, labeling CDC42 with ab155940 at 1/20 dilution. Detection was performed using a biotin conjugated secondary antibody and SA-HRP. Right image is immunohistochemistry performed without primary antibody (negative control).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab155940 has been referenced in 12 publications.
- Emmrich S et al. Characterization of naked mole-rat hematopoiesis reveals unique stem and progenitor cell patterns and neotenic traits. EMBO J 41:e109694 (2022). PubMed: 35694726
- Yang TY et al. Mulberry Leaf and Neochlorogenic Acid Alleviates Glucolipotoxicity-Induced Oxidative Stress and Inhibits Proliferation/Migration via Downregulating Ras and FAK Signaling Pathway in Vascular Smooth Muscle Cell. Nutrients 14:N/A (2022). PubMed: 35893859
- Cui Q et al. Leptin Promotes Angiogenesis via Pericyte STAT3 Pathway upon Intracerebral Hemorrhage. Cells 11:N/A (2022). PubMed: 36078162
- Pickering KA et al. A RAC-GEF network critical for early intestinal tumourigenesis. Nat Commun 12:56 (2021). PubMed: 33397922
- Lin J et al. MiR-23a induced the activation of CDC42/PAK1 pathway and cell cycle arrest in human cov434 cells by targeting FGD4. J Ovarian Res 13:90 (2020). PubMed: 32772928