• Product name
  • Description
    Rabbit polyclonal to CDC42
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Saccharomyces cerevisiae
    Predicted to work with: Pig, Chimpanzee, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human CDC42.

    (Peptide available as ab66571.)

  • Positive control
    • This antibody gave a positive signal in the following Human Whole Cell Lysates: HeLa, Jurkat, HepG2, MCF7, U2OS, A431 (data not shown), HEK 293 (data not shown) and SHSY-5Y (data not shown)



Our Abpromise guarantee covers the use of ab64533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IHC-Fr 1/100.
IHC-P Use at an assay dependent concentration.


  • Function
    Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
  • Post-translational
    AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Form
    There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: Brain; Isoform 2 also known as: Placental.
  • Alternative names
    • CDC42 antibody
    • CDC42_HUMAN antibody
    • CDC42Hs antibody
    • Cell division control protein 42 homolog antibody
    • Cell division cycle 42 (GTP binding protein 25kDa) antibody
    • Cell division cycle 42 antibody
    • dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody
    • dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody
    • G25K antibody
    • G25K GTP-binding protein antibody
    • Growth regulating protein antibody
    • GTP binding protein 25kDa antibody
    • Small GTP binding protein CDC42 antibody
    • TKS antibody
    see all


  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: CDC42 knockout HAP1 cell lysate (20 µg)
    Lane 3: MCF7 cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green – ab64533 observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa.

    Ab64533 was shown to recognize CDC42 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when CDC42 knockout samples were eexamined. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. Ab64533 and ab8245 (loading control to GAPDH) were diluted at 1µg/ml and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-CDC42 antibody (ab64533) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 5 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21 kDa
    Observed band size: 21 kDa
    Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 3 minutes
  • All lanes : Anti-CDC42 antibody (ab64533) at 1 µg/ml

    Lane 1 : Recombinant Human CDC42 protein (ab87713) at 0.1 µg
    Lane 2 : Recombinant Human CDC42 protein (ab87713) at 0.01 µg

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21 kDa

    Exposure time: 1 minute
  • ICC/IF image of ab64533 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64533, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% pfa fixed (10 min) HeLa cells at 5µg/ml.


This product has been referenced in:
  • Brooks AB  et al. MYO6 is targeted bySalmonellavirulence effectors to trigger PI3-kinase signaling and pathogen invasion into host cells. Proc Natl Acad Sci U S A 114:3915-3920 (2017). Read more (PubMed: 28348208) »
  • Druso JE  et al. An Essential Role for Cdc42 in the Functioning of the Adult Mammary Gland. J Biol Chem 291:8886-95 (2016). Read more (PubMed: 26912661) »

See all 11 Publications for this product

Customer reviews and Q&As

The nucleotide binding sites of this protein correspond to amino acids 10-17, 57-61 and 115-118.
Since the immunogen of this antibody corresponds to amino acids xxxxxxx, I can confirm that this antibody will not be able to distinguish between GTP ...

Read More

ab41429 does not detect RhoA, we have tested this directly using recombinant RhoA protein versus recombinant CDC42 protein. However, we have not tested reactivity to Rac1 and therefore, we cannot comment on the cross-reactivity with Rac1 since the epit...

Read More


Sign up