Anti-CDC42 Binding Protein Kinase Beta antibody (ab61328)

Overview

  • Product name
    Anti-CDC42 Binding Protein Kinase Beta antibody
    See all CDC42 Binding Protein Kinase Beta primary antibodies
  • Description
    Mouse monoclonal to CDC42 Binding Protein Kinase Beta
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment with tag: SKMISNPTNF NHVAHMGPGD GMQVLMDLPL SAVPPSQEER PGPAPTNLAR QPPSRNKPYI SWPSSGGSEP SVTVPLRSMS DPDQDFDKEP DSDSTKHSTP , corresponding to amino acids 1580-1679 of Human CDC42 Binding Protein Kinase Beta

  • Positive control
    • Jurkat cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 194 kDa (predicted molecular weight: 194 kDa).
ELISA Use at an assay dependent dilution.

Target

  • Function
    May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.
  • Tissue specificity
    Expressed in all tissues examined, with high levels in heart, brain, placenta and lung.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 CNH domain.
    Contains 1 CRIB domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • Cellular localization
    Cytoplasm. Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC42 binding protein kinase beta antibody
    • CDC42-binding protein kinase beta antibody
    • CDC42BPB antibody
    • DMPK like beta antibody
    • DMPK-like beta antibody
    • KIAA1124 antibody
    • MRCK beta antibody
    • MRCKB_HUMAN antibody
    • Myotonic dystrophy kinase related CDC42 binding kinase beta antibody
    • Myotonic dystrophy kinase-related CDC42-binding kinase beta antibody
    • Myotonic dystrophy protein kinase like beta antibody
    • Myotonic dystrophy protein kinase-like beta antibody
    • Serine/threonine protein kinase MRCK beta antibody
    • Serine/threonine-protein kinase MRCK beta antibody
    see all

Anti-CDC42 Binding Protein Kinase Beta antibody images

  • Anti-CDC42 Binding Protein Kinase Beta antibody (ab61328) at 1 µg/ml + Jurkat whole cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 194 kDa
    Observed band size : 194 kDa
  • All lanes : Anti-CDC42 Binding Protein Kinase Beta antibody (ab61328) at 1/2000 dilution

    Lane 1 : Cytosolic fraction prepared from murine brain tissue
    Lane 2 : Cytosolic fraction prepared from murine brain tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) (ab6789) at 1/2000 dilution
    Developed using the ECL technique

    Predicted band size : 194 kDa


    Exposure time : 1 minute

    Image courtesy of an anonymous Abreview.

    See Abreview

References for Anti-CDC42 Binding Protein Kinase Beta antibody (ab61328)

ab61328 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (brain tissue lysate, cytosilic fraction)
Loading amount
10 µg
Specification
brain tissue lysate, cytosilic fraction
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Abcam user community

Verified customer

Submitted Apr 05 2012

Thank you for your reply.
Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these addition...

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