Anti-CDC42 Binding Protein Kinase Beta antibody (ab69589)

Overview

  • Product nameAnti-CDC42 Binding Protein Kinase Beta antibody
    See all CDC42 Binding Protein Kinase Beta primary antibodies
  • Description
    Rabbit polyclonal to CDC42 Binding Protein Kinase Beta
  • SpecificityDetects endogenous levels of total CDC42 Binding Protein Kinase Beta protein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide from an internal of human MRCKB.

  • Positive control
    • Human brain tissue COLO cell extract

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 194 kDa (predicted molecular weight: 194 kDa).
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionMay act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.
  • Tissue specificityExpressed in all tissues examined, with high levels in heart, brain, placenta and lung.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 CNH domain.
    Contains 1 CRIB domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • Cellular localizationCytoplasm. Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC42 binding protein kinase beta antibody
    • CDC42-binding protein kinase beta antibody
    • CDC42BPB antibody
    • DMPK like beta antibody
    • DMPK-like beta antibody
    • KIAA1124 antibody
    • MRCK beta antibody
    • MRCKB_HUMAN antibody
    • Myotonic dystrophy kinase related CDC42 binding kinase beta antibody
    • Myotonic dystrophy kinase-related CDC42-binding kinase beta antibody
    • Myotonic dystrophy protein kinase like beta antibody
    • Myotonic dystrophy protein kinase-like beta antibody
    • Serine/threonine protein kinase MRCK beta antibody
    • Serine/threonine-protein kinase MRCK beta antibody
    see all

Anti-CDC42 Binding Protein Kinase Beta antibody images

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab69589 at 1/50-1/100 dilution. Right hand panel was treated with immunising peptide.
  • All lanes : Anti-CDC42 Binding Protein Kinase Beta antibody (ab69589) at 1/500 dilution

    Lane 1 : COLO cell extract
    Lane 2 : COLO cell extract with peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 194 kDa
    Observed band size : 194 kDa

References for Anti-CDC42 Binding Protein Kinase Beta antibody (ab69589)

ab69589 has not yet been referenced specifically in any publications.

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