Overview

  • Product name
    Anti-CDC42EP5 antibody
  • Description
    Rabbit polyclonal to CDC42EP5
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide (Human) from the N-terminus of CDC42EP5.

  • Positive control
    • Jurkat cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab75004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 15 kDa).
ELISA 1/40000.

Target

  • Relevance
    CDC42EP5 belongs to a family of CDC42 binding proteins that induce actin filament assembly, leading to changes in cell shape.
  • Cellular localization
    Cytoplasm. Cytoskeleton. Membrane; extrinsic to membrane.
  • Database links
  • Alternative names
    • Binder of Rho GTPase 3 like antibody
    • Binder of Rho GTPases 3 antibody
    • BORG3 antibody
    • CDC42 effector protein (Rho GTPase binding) 5 antibody
    • CDC42 effector protein 5 antibody
    • CEP5 antibody
    see all

Images

  • All lanes : Anti-CDC42EP5 antibody (ab75004) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 15 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.

References

ab75004 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab75004.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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