Overview

  • Product name
    Anti-CDC42SE2 antibody
  • Description
    Rabbit polyclonal to CDC42SE2
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence: EPTNFVHTAH VGSGDLFSGM NSVSSIQNQM QSKGGYGGGM PANVQMQLVD TKAG, corresponding to amino acids 31-84 of Human CDC42SE2.

  • Positive control
    • RT 4, U 251 MG, Human Liver and Human Tonsil lysates; Human small intestine tissue; A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab122492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

Target

  • Function
    Probably involved in the organization of the actin cytoskeleton by acting downstream of CDC42, inducing actin filament assembly. Alters CDC42-induced cell shape changes. In activated T-cells, may play a role in CDC42-mediated F-actin accumulation at the immunological synapse. May play a role in early contractile events in phagocytosis in macrophages.
  • Tissue specificity
    Widely expressed. Expressed at higher level in T lymphocytes. Highly expressed in CCRF-CEM T lymphocytes, Jurkat T lymphocytes, and Raji B lymphocytes compared (at protein level).
  • Sequence similarities
    Belongs to the CDC42SE/SPEC family.
    Contains 1 CRIB domain.
  • Domain
    The CRIB domain mediates interaction with CDC42.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell membrane. Cell projection > phagocytic cup. Recruited to the activated TCR prior actin polymerization. Localizes at the phagocytic cup of macrophages.
  • Information by UniProt
  • Database links
  • Alternative names
    • C42S2_HUMAN antibody
    • CDC42 small effector protein 2 antibody
    • Cdc42se2 antibody
    • FLJ21967 antibody
    • Small effector of CDC42 protein 2 antibody
    • SPEC2 antibody
    see all

Images

  • All lanes : Anti-CDC42SE2 antibody (ab122492) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique
  • ab122492 at 1/50 dilution staining CDC42SE2 in Paraffin-embedded Human small intestine tissue by Immunohistochemistry.
  • ab122492 at 4 µg/ml staining CDC42SE2 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).

References

ab122492 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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