Anti-CDC45L antibody (ab230178)
Key features and details
- Rabbit polyclonal to CDC45L
- Suitable for: WB, ICC/IF, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CDC45L antibody
See all CDC45L primary antibodies -
Description
Rabbit polyclonal to CDC45L -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CDC45L aa 1-270.
Database link: O75419 -
Positive control
- WB: K562, HEK-293T, HeLa and HepG2 whole cell lysate. IHC-P: Human kidney tissue. IP: K562 whole cell lysate. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 66, 58 kDa (predicted molecular weight: 66, 60, 69 kDa).
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ICC/IF |
1/50 - 1/200.
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IP |
1/20 - 1/2000.
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 66, 58 kDa (predicted molecular weight: 66, 60, 69 kDa). |
ICC/IF
1/50 - 1/200. |
IP
1/20 - 1/2000. |
IHC-P
1/20 - 1/200. |
Target
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Function
Required for initiation of chromosomal DNA replication. -
Tissue specificity
Widely expressed, highest levels are found in adult testis and thymus and in fetal liver. -
Sequence similarities
Belongs to the CDC45 family. -
Developmental stage
Transcript peaks at G1-S transition, but total protein remains constant throughout the cell cycle. Expressed in multiple tissues during embryogenesis, including neural crest-derived structures. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8318 Human
- Omim: 603465 Human
- SwissProt: O75419 Human
- Unigene: 474217 Human
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Alternative names
- CDC 45 antibody
- CDC 45L antibody
- CDC45 (cell division cycle 45, S.cerevisiae homolog) like antibody
see all
Images
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All lanes : Anti-CDC45L antibody (ab230178) at 1/1000 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 66, 60, 69 kDa
Observed band size: 58,66 kDa why is the actual band size different from the predicted? -
Paraffin-embedded human kidney tissue stained for CDC45L with ab230178 at 1/100 dilution in immunohistochemical analysis.
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CDC45L was immunoprecipitated from K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate using ab230178.
Lane 1: Control IgG IP in K562 whole cell lysate.
Lane 2: ab230178 in K562 whole cell lysate.
Lane 3: CDC45L in K562 whole cell lysate (Input).
For western blotting, an HRP-conjugated light chain specific antibody was used as the secondary antibody (1/50,000).
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CDC45L using ab230178 at a dilution of 1/100 in ICC/IF.
Secondary is an Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab230178 has not yet been referenced specifically in any publications.