The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
FunctionRequired for initiation of chromosomal DNA replication.
Tissue specificityWidely expressed, highest levels are found in adult testis and thymus and in fetal liver.
Sequence similaritiesBelongs to the CDC45 family.
Developmental stageTranscript peaks at G1-S transition, but total protein remains constant throughout the cell cycle. Expressed in multiple tissues during embryogenesis, including neural crest-derived structures.