Overview

  • Product nameAnti-CDC45L antibody
    See all CDC45L primary antibodies
  • Description
    Rabbit polyclonal to CDC45L
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 504-553 (VTVVGIPPET DSSDRKNFFG RAFEKAAEST SSRMLHNHFD LSVIELKAED) of Human CDC45L (NP_003495).

  • Positive control
    • Hela cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab82805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/12500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionRequired for initiation of chromosomal DNA replication.
  • Tissue specificityWidely expressed, highest levels are found in adult testis and thymus and in fetal liver.
  • Sequence similaritiesBelongs to the CDC45 family.
  • Developmental stageTranscript peaks at G1-S transition, but total protein remains constant throughout the cell cycle. Expressed in multiple tissues during embryogenesis, including neural crest-derived structures.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC 45 antibody
    • CDC 45L antibody
    • CDC45 (cell division cycle 45, S.cerevisiae homolog) like antibody
    • CDC45 antibody
    • CDC45 cell division cycle 45 like antibody
    • CDC45 like antibody
    • CDC45 related protein antibody
    • CDC45_HUMAN antibody
    • CDC45L2 antibody
    • Cell division control protein 45 homolog antibody
    • Cell division cycle 45 like 2 antibody
    • Cell division cycle 45 like antibody
    • PORC PI 1 antibody
    • PORC-PI-1 antibody
    see all

Anti-CDC45L antibody images

  • Anti-CDC45L antibody (ab82805) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 66 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa,>90 kDa. We are unsure as to the identity of these extra bands.

References for Anti-CDC45L antibody (ab82805)

ab82805 has not yet been referenced specifically in any publications.

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