Overview

  • Product name
    Anti-CDC45L antibody [EPR5758]
    See all CDC45L primary antibodies
  • Description
    Rabbit monoclonal [EPR5758] to CDC45L
  • Tested applications
    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human CDC45L.

  • Positive control
    • HeLa, Jurkat, K562, and 293T cell lysates. Human kidney tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab108350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa).
IHC-P 1/50 - 1/100. Perform antigen retrieval before commencing with IHC staining protocol
ICC 1/50 - 1/100.
Flow Cyt 1/500 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Required for initiation of chromosomal DNA replication.
    • Tissue specificity
      Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.
    • Sequence similarities
      Belongs to the CDC45 family.
    • Developmental stage
      Transcript peaks at G1-S transition, but total protein remains constant throughout the cell cycle. Expressed in multiple tissues during embryogenesis, including neural crest-derived structures.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CDC 45 antibody
      • CDC 45L antibody
      • CDC45 (cell division cycle 45, S.cerevisiae homolog) like antibody
      • CDC45 antibody
      • CDC45 cell division cycle 45 like antibody
      • CDC45 like antibody
      • CDC45 related protein antibody
      • CDC45_HUMAN antibody
      • CDC45L2 antibody
      • Cell division control protein 45 homolog antibody
      • Cell division cycle 45 like 2 antibody
      • Cell division cycle 45 like antibody
      • PORC PI 1 antibody
      • PORC-PI-1 antibody
      see all

    Images

    • All lanes : Anti-CDC45L antibody [EPR5758] (ab108350) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 66 kDa
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab108350 at 1/50
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Anantha RW  et al. Requirement of heterogeneous nuclear ribonucleoprotein C for BRCA gene expression and homologous recombination. PLoS One 8:e61368 (2013). WB . Read more (PubMed: 23585894) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab108350.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up