The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa).
1/50 - 1/100. Perform antigen retrieval before commencing with IHC staining protocol
1/50 - 1/100.
1/500 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Application notesIs unsuitable for IP.
FunctionRequired for initiation of chromosomal DNA replication.
Tissue specificityWidely expressed, highest levels are found in adult testis and thymus and in fetal liver.
Sequence similaritiesBelongs to the CDC45 family.
Developmental stageTranscript peaks at G1-S transition, but total protein remains constant throughout the cell cycle. Expressed in multiple tissues during embryogenesis, including neural crest-derived structures.