Overview

  • Product nameAnti-CDC5L antibody [2136C1a]
    See all CDC5L primary antibodies
  • Description
    Mouse monoclonal [2136C1a] to CDC5L
  • Tested applicationsSuitable for: Flow Cyt, WB, IP, ICC, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant human CDC5L fragment

  • Positive control
    • HeLa cells and whole cell lysate, NIH3T3 whole cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, 8mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number2136C1a
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/100. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
IP Use at 10 µg/mg of lysate. RIPA lysate of HeLa cells
ICC 1/10.
Dot blot Use at an assay dependent concentration.

Target

  • FunctionDNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.
  • Tissue specificityUbiquitously expressed in both fetal and adult tissues.
  • Involvement in diseaseA chromosomal aberration involving CDC5L is found in multicystic renal dysplasia. Translocation t(6;19)(p21;q13.1) with USF2.
  • Sequence similaritiesBelongs to the CEF1 family.
    Contains 2 HTH myb-type DNA-binding domains.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8.
  • Cellular localizationNucleus. Nucleus speckle. Cytoplasm. May shuttle between cytoplasm and nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC5 antibody
    • CDC5 cell division cycle 5-like (S. pombe) antibody
    • Cdc5 related protein antibody
    • CDC5-LIKE antibody
    • Cdc5-like protein antibody
    • Cdc5l antibody
    • CDC5L_HUMAN antibody
    • CEF1 antibody
    • Cell division cycle 5 S. pombe homolog of antibody
    • Cell division cycle 5-like protein antibody
    • dJ319D22.1 antibody
    • KIAA0432 antibody
    • PCDC5RP antibody
    • Pombe Cdc5 related protein antibody
    • Pombe Cdc5-related protein antibody
    see all

Anti-CDC5L antibody [2136C1a] images

  • Anti-CDC5L antibody [2136C1a] (ab51320) at 1/100 dilution + HeLa whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 89 kDa
    Observed band size : 91 kDa (why is the actual band size different from the predicted?)
  • ab51320 at 1/10 dilution staining human CDC5L in HeLa cells by Immunocytochemistry. 1/200 dilution AlexaFluor488 Goat Anti-mouse IgG was used as a secondary antibody.
  • Anti-CDC5L antibody [2136C1a] (ab51320) at 1/100 dilution + NIH3T3 whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 89 kDa
    Observed band size : 89 kDa
  • Immunoprecipitation analysis using ab51320 at 5ug/500ug RIPA lysate of HeLa cells. Immunoblot uses ab51320 at 1/100 dilution and anti-mouse IgG at 1/2500 as secondary.
  • Overlay histogram showing HeLa cells stained with ab51320 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51320, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-CDC5L antibody [2136C1a] (ab51320)

This product has been referenced in:
  • Guilgur LG  et al. Requirement for highly efficient pre-mRNA splicing during Drosophila early embryonic development. Elife 3:e02181 (2014). WB . Read more (PubMed: 24755291) »
  • Sousa MM  et al. An inverse switch in DNA base excision and strand break repair contributes to melphalan resistance in multiple myeloma cells. PLoS One 8:e55493 (2013). WB ; Human . Read more (PubMed: 23405159) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"