The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
1/100. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
Use at 10 µg/mg of lysate. RIPA lysate of HeLa cells
Use at an assay dependent concentration.
FunctionDNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.
Tissue specificityUbiquitously expressed in both fetal and adult tissues.
Involvement in diseaseA chromosomal aberration involving CDC5L is found in multicystic renal dysplasia. Translocation t(6;19)(p21;q13.1) with USF2.
Sequence similaritiesBelongs to the CEF1 family. Contains 2 HTH myb-type DNA-binding domains.
Post-translational modificationsPhosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8.
Cellular localizationNucleus. Nucleus speckle. Cytoplasm. May shuttle between cytoplasm and nucleus.
Overlay histogram showing HeLa cells stained with ab51320 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51320, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-CDC5L antibody [2136C1a] (ab51320)
This product has been referenced in:
Guilgur LG et al. Requirement for highly efficient pre-mRNA splicing during Drosophila early embryonic development. Elife3:e02181 (2014).
Read more (PubMed: 24755291) »
Sousa MM et al. An inverse switch in DNA base excision and strand break repair contributes to melphalan resistance in multiple myeloma cells. PLoS One8:e55493 (2013).
Read more (PubMed: 23405159) »