Overview

  • Product name
  • Description
    Rabbit polyclonal to CDC5L
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 262-536 of Human CDC5L (Uniprot ID: Q99459).

  • Positive control
    • 293T, Jurkat and Mouse brain whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 92 kDa.

Target

  • Function
    DNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.
  • Tissue specificity
    Ubiquitously expressed in both fetal and adult tissues.
  • Involvement in disease
    A chromosomal aberration involving CDC5L is found in multicystic renal dysplasia. Translocation t(6;19)(p21;q13.1) with USF2.
  • Sequence similarities
    Belongs to the CEF1 family.
    Contains 2 HTH myb-type DNA-binding domains.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8.
  • Cellular localization
    Nucleus. Nucleus speckle. Cytoplasm. May shuttle between cytoplasm and nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC5 antibody
    • CDC5 cell division cycle 5-like (S. pombe) antibody
    • Cdc5 related protein antibody
    • CDC5-LIKE antibody
    • Cdc5-like protein antibody
    • Cdc5l antibody
    • CDC5L_HUMAN antibody
    • CEF1 antibody
    • Cell division cycle 5 S. pombe homolog of antibody
    • Cell division cycle 5-like protein antibody
    • dJ319D22.1 antibody
    • KIAA0432 antibody
    • PCDC5RP antibody
    • Pombe Cdc5 related protein antibody
    • Pombe Cdc5-related protein antibody
    see all

Images

  • Anti-CDC5L antibody (ab155914) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size: 92 kDa



    7.5% SDS PAGE
  • Anti-CDC5L antibody (ab155914) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 92 kDa



    7.5% SDS PAGE

References

ab155914 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab155914.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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