Overview

  • Product nameAnti-CDC5L antibody
    See all CDC5L primary antibodies
  • Description
    Rabbit polyclonal to CDC5L
  • Tested applicationsSuitable for: IP, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide derived from within residues 750 to the C-terminus of Human CDC5L.

    (Peptide available as ab30674.)

  • Positive control
    • HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate ; Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate; A431 (Human epithelial carcinoma cell line) Whole Cell Lysate;

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
ICC/IF
IHC-P
  • Application notesICC/IF: Use at a concentration of 1 µg/ml.
    IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
    IP: Use at an assay dependent dilution (PMID 18722174).
    WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 93 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.
    • Tissue specificityUbiquitously expressed in both fetal and adult tissues.
    • Involvement in diseaseA chromosomal aberration involving CDC5L is found in multicystic renal dysplasia. Translocation t(6;19)(p21;q13.1) with USF2.
    • Sequence similaritiesBelongs to the CEF1 family.
      Contains 2 HTH myb-type DNA-binding domains.
    • Post-translational
      modifications
      Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8.
    • Cellular localizationNucleus. Nucleus speckle. Cytoplasm. May shuttle between cytoplasm and nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CDC5 antibody
      • CDC5 cell division cycle 5-like (S. pombe) antibody
      • Cdc5 related protein antibody
      • CDC5-LIKE antibody
      • Cdc5-like protein antibody
      • Cdc5l antibody
      • CDC5L_HUMAN antibody
      • CEF1 antibody
      • Cell division cycle 5 S. pombe homolog of antibody
      • Cell division cycle 5-like protein antibody
      • dJ319D22.1 antibody
      • KIAA0432 antibody
      • PCDC5RP antibody
      • Pombe Cdc5 related protein antibody
      • Pombe Cdc5-related protein antibody
      see all

    Anti-CDC5L antibody images

    • All lanes : Anti-CDC5L antibody (ab31779) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat whole cell lysate (ab7899)
      Lane 3 : A431 whole cell lysate (ab7909)

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 93 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 50 kDa (possible cleavage fragment,cross reactivity),55 kDa (possible cleavage fragment,cross reactivity).
    • ICC/IF image of ab31779 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31779, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
    • Image courtesy of Human Protein Atlas

      ab31779 staining in human stomach, showing staining of the glanular cells (in brown). Paraffin embedded lower stomach tissue was incubated with ab31779 (1:60 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab31779 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

      .

    References for Anti-CDC5L antibody (ab31779)

    This product has been referenced in:
    • van Maldegem F  et al. CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex. Nucleic Acids Res 43:7058-69 (2015). ICC/IF ; Mouse . Read more (PubMed: 26130721) »
    • David CJ  et al. The RNA polymerase II C-terminal domain promotes splicing activation through recruitment of a U2AF65-Prp19 complex. Genes Dev 25:972-83 (2011). IP . Read more (PubMed: 21536736) »

    See all 3 Publications for this product

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"