• Product nameAnti-CDC5L antibody
    See all CDC5L primary antibodies
  • Description
    Rabbit polyclonal to CDC5L
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the C terminal amino acids 753-802 (EELKKHEDSA IPRRLECLKE DVQRQQEREK ELQHRYADLL LEKETLKSKF) of human CDC5L (NP_001244).

  • Positive control
    • 721_B cell lysate


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab86971 is purified by peptide affinity chromatography method.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab86971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 92 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • FunctionDNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.
  • Tissue specificityUbiquitously expressed in both fetal and adult tissues.
  • Involvement in diseaseA chromosomal aberration involving CDC5L is found in multicystic renal dysplasia. Translocation t(6;19)(p21;q13.1) with USF2.
  • Sequence similaritiesBelongs to the CEF1 family.
    Contains 2 HTH myb-type DNA-binding domains.
  • Post-translational
    Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8.
  • Cellular localizationNucleus. Nucleus speckle. Cytoplasm. May shuttle between cytoplasm and nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC5 antibody
    • CDC5 cell division cycle 5-like (S. pombe) antibody
    • Cdc5 related protein antibody
    • CDC5-LIKE antibody
    • Cdc5-like protein antibody
    • Cdc5l antibody
    • CDC5L_HUMAN antibody
    • CEF1 antibody
    • Cell division cycle 5 S. pombe homolog of antibody
    • Cell division cycle 5-like protein antibody
    • dJ319D22.1 antibody
    • KIAA0432 antibody
    • PCDC5RP antibody
    • Pombe Cdc5 related protein antibody
    • Pombe Cdc5-related protein antibody
    see all

Anti-CDC5L antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling CDC5L with ab86971 at 4.0-8.0µg/ml. Positive staining shown in alveolar cells. Magnification: 400X.
  • Anti-CDC5L antibody (ab86971) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 92 kDa
    Observed band size : ~89 kDa (why is the actual band size different from the predicted?)
    Additional bands at : <40 kDa,~50 kDa. We are unsure as to the identity of these extra bands.
    6-18% gel

References for Anti-CDC5L antibody (ab86971)

ab86971 has not yet been referenced specifically in any publications.

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