Recombinant
RabMAb

Anti-Cdc6 antibody [EPR714(2)] (ab109315)

Overview

  • Product name
    Anti-Cdc6 antibody [EPR714(2)]
    See all Cdc6 primary antibodies
  • Description
    Rabbit monoclonal [EPR714(2)] to Cdc6
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues on the N terminus in Human Cdc6.

  • Positive control
    • WB: Hela treated with hydroxyurea and Raji treated with FBS lysates IHC-P: Human gastric adenocarcinoma and Human breast carcinoma tissue
  • General notes

     

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 63 kDa).
IHC-P 1/1000 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
    • Sequence similarities
      Belongs to the CDC6/cdc18 family.
    • Cellular localization
      Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cdc 18L antibody
      • Cdc 6 antibody
      • CDC18 (cell division cycle 18, S.pombe, homolog) like antibody
      • CDC18 (S.pombe) antibody
      • Cdc18 related protein antibody
      • CDC18(S.pombe) antibody
      • Cdc18-related protein antibody
      • Cdc18L antibody
      • cdc6 antibody
      • CDC6 cell division cycle 6 homolog antibody
      • CDC6 related protein antibody
      • CDC6-related protein antibody
      • CDC6_HUMAN antibody
      • Cdc6p antibody
      • CELL CYCLE CONTROLLER CDC6 antibody
      • Cell division control protein 6 antibody
      • Cell division control protein 6 homolog antibody
      • Cell division cycle 6 homolog (S. cerevisiae) antibody
      • Cell division cycle 6 homolog antibody
      • Cell division cycle 6, S. cerevisiae, homolog of antibody
      • HsCDC 6 antibody
      • HsCDC18 antibody
      • HsCDC6 antibody
      • p62 antibody
      • p62(cdc 6) antibody
      • p62(cdc6) antibody
      see all

    Images

    • All lanes : Anti-Cdc6 antibody [EPR714(2)] (ab109315) at 1/10000 dilution

      Lane 1 : Hela treated with hydroxyurea lysate
      Lane 2 : Raji treated with FBS lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 63 kDa
      Observed band size : 62 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of Cdc6 in paraffin embedded Human gastric adenocarcinoma tissue, using ab109315 at a 1/1000 dilution.
    • Immunohistochemical analysis of Cdc6 in paraffin embedded Human breast carcinoma tissue, using ab109315 at a 1/1000 dilution.

    References

    This product has been referenced in:
    • He Y  et al. Cell Division Cycle 6 Promotes Mitotic Slippage and Contributes to Drug Resistance in Paclitaxel-Treated Cancer Cells. PLoS One 11:e0162633 (2016). WB ; Human . Read more (PubMed: 27611665) »
    • Huang J  et al. miR-34a Modulates Angiotensin II-Induced Myocardial Hypertrophy by Direct Inhibition of ATG9A Expression and Autophagic Activity. PLoS One 9:e94382 (2014). WB ; Rat . Read more (PubMed: 24728149) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Aug 23 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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