Recombinant
RabMAb

Anti-Cdc6 antibody [EPR714(2)] (ab109315)

Overview

  • Product name
    Anti-Cdc6 antibody [EPR714(2)]
    See all Cdc6 primary antibodies
  • Description
    Rabbit monoclonal [EPR714(2)] to Cdc6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cdc6 aa 1-100 (N terminal). The exact sequence is proprietary.
    (Peptide available as ab197759)

  • Positive control
    • WB: Hela treated with hydroxyurea and Raji treated with FBS lysates IHC-P: Human gastric adenocarcinoma and Human breast carcinoma tissue
  • General notes

     

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 63 kDa).
IHC-P 1/1000 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
    • Sequence similarities
      Belongs to the CDC6/cdc18 family.
    • Cellular localization
      Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cdc 18L antibody
      • Cdc 6 antibody
      • CDC18 (cell division cycle 18, S.pombe, homolog) like antibody
      • CDC18 (S.pombe) antibody
      • Cdc18 related protein antibody
      • CDC18(S.pombe) antibody
      • Cdc18-related protein antibody
      • Cdc18L antibody
      • cdc6 antibody
      • CDC6 cell division cycle 6 homolog antibody
      • CDC6 related protein antibody
      • CDC6-related protein antibody
      • CDC6_HUMAN antibody
      • Cdc6p antibody
      • CELL CYCLE CONTROLLER CDC6 antibody
      • Cell division control protein 6 antibody
      • Cell division control protein 6 homolog antibody
      • Cell division cycle 6 homolog (S. cerevisiae) antibody
      • Cell division cycle 6 homolog antibody
      • Cell division cycle 6, S. cerevisiae, homolog of antibody
      • HsCDC 6 antibody
      • HsCDC18 antibody
      • HsCDC6 antibody
      • p62 antibody
      • p62(cdc 6) antibody
      • p62(cdc6) antibody
      see all

    Images

    • All lanes : Anti-Cdc6 antibody [EPR714(2)] (ab109315) at 1/10000 dilution

      Lane 1 : Hela treated with hydroxyurea lysate
      Lane 2 : Raji treated with FBS lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 63 kDa
      Observed band size: 62 kDa (why is the actual band size different from the predicted?)

    • Immunohistochemical analysis of Cdc6 in paraffin embedded Human gastric adenocarcinoma tissue, using ab109315 at a 1/1000 dilution.
    • Immunohistochemical analysis of Cdc6 in paraffin embedded Human breast carcinoma tissue, using ab109315 at a 1/1000 dilution.

    References

    This product has been referenced in:
    • He Y  et al. Cell Division Cycle 6 Promotes Mitotic Slippage and Contributes to Drug Resistance in Paclitaxel-Treated Cancer Cells. PLoS One 11:e0162633 (2016). WB ; Human . Read more (PubMed: 27611665) »
    • Huang J  et al. miR-34a Modulates Angiotensin II-Induced Myocardial Hypertrophy by Direct Inhibition of ATG9A Expression and Autophagic Activity. PLoS One 9:e94382 (2014). WB ; Rat . Read more (PubMed: 24728149) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Aug 23 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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