For the best experience on the Abcam website please upgrade to a modern browser such as
Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab53689 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Corresponding non-phospho antibody
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
Function Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Sequence similarities Belongs to the CDC6/cdc18 family.
Cellular localization Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells.
Information by UniProt
Cdc 18L antibody
Cdc 6 antibody
CDC18 (cell division cycle 18, S.pombe, homolog) like antibody
Anti-Cdc6 (phospho S54) antibody images
Western blot - Cdc6 (phospho S54) antibody (ab53689)
All lanes : Anti-Cdc6 (phospho S54) antibody (ab53689) at 1/500 dilution Lane 1 : COS7 cell extract treated
with EGF (200ng/ml, 30 mins) Lane 2 : COS7 cell extract treated
with EGF (200ng/ml, 30 mins) with immunising peptide Predicted band size : 63 kDa Observed band size : 63 kDa
References for Anti-Cdc6 (phospho S54) antibody (ab53689)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"