Overview

  • Product name
    Anti-Cdc6 (phospho S54) antibody
    See all Cdc6 primary antibodies
  • Description
    Rabbit polyclonal to Cdc6 (phospho S54)
  • Specificity
    ab53689 detects endogenous levels of Cdc6 only when phosphorylated at serine 54.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human Cdc6 around the phosphorylation site of serine 54 (P-L-SP-P-R).

  • Positive control
    • COS7 cell extracts treated with EGF (200ng/ml, 30 mins).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab53689 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
ELISA 1/5000.

Target

  • Function
    Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
  • Sequence similarities
    Belongs to the CDC6/cdc18 family.
  • Cellular localization
    Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cdc 18L antibody
    • Cdc 6 antibody
    • CDC18 (cell division cycle 18, S.pombe, homolog) like antibody
    • CDC18 (S.pombe) antibody
    • Cdc18 related protein antibody
    • CDC18(S.pombe) antibody
    • Cdc18-related protein antibody
    • Cdc18L antibody
    • cdc6 antibody
    • CDC6 cell division cycle 6 homolog antibody
    • CDC6 related protein antibody
    • CDC6-related protein antibody
    • CDC6_HUMAN antibody
    • Cdc6p antibody
    • CELL CYCLE CONTROLLER CDC6 antibody
    • Cell division control protein 6 antibody
    • Cell division control protein 6 homolog antibody
    • Cell division cycle 6 homolog (S. cerevisiae) antibody
    • Cell division cycle 6 homolog antibody
    • Cell division cycle 6, S. cerevisiae, homolog of antibody
    • HsCDC 6 antibody
    • HsCDC18 antibody
    • HsCDC6 antibody
    • p62 antibody
    • p62(cdc 6) antibody
    • p62(cdc6) antibody
    see all

Anti-Cdc6 (phospho S54) antibody images

  • All lanes : Anti-Cdc6 (phospho S54) antibody (ab53689) at 1/500 dilution

    Lane 1 : COS7 cell extract treated with EGF (200ng/ml, 30 mins)
    Lane 2 : COS7 cell extract treated with EGF (200ng/ml, 30 mins) with immunising peptide


    Predicted band size : 63 kDa
    Observed band size : 63 kDa

References for Anti-Cdc6 (phospho S54) antibody (ab53689)

ab53689 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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