Anti-Cdc7 Kinase antibody (ab113956)

Overview

  • Product nameAnti-Cdc7 Kinase antibody
    See all Cdc7 Kinase primary antibodies
  • Description
    Rabbit polyclonal to Cdc7 Kinase
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Guinea pig, Cow, Dog, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 524 and 574 of Human Cdc7 Kinase (NP_003494.1).

  • Positive control
    • Whole cell lysate from 293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab113956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 64 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionSeems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cdc 7 antibody
    • CDC7 antibody
    • CDC7 cell division cycle 7 antibody
    • CDC7 cell division cycle 7 like 1 antibody
    • CDC7 L1 antibody
    • Cdc7 like 1 antibody
    • CDC7 related kinase antibody
    • CDC7-related kinase antibody
    • CDC7_HUMAN antibody
    • CDC7L 1 antibody
    • CDC7L1 antibody
    • Cell division cycle 7 homolog antibody
    • Cell division cycle 7 kinase antibody
    • Cell division cycle 7 like protein 1 antibody
    • Cell division cycle 7 related protein kinase antibody
    • Cell division cycle 7-related protein kinase antibody
    • HsCDC 7 antibody
    • HsCdc7 antibody
    • Hsk 1 antibody
    • Hsk1 antibody
    • HuCDC 7 antibody
    • huCdc7 antibody
    • MGC117361 antibody
    • MGC126237 antibody
    • MGC126238 antibody
    see all

Anti-Cdc7 Kinase antibody images

  • All lanes : Anti-Cdc7 Kinase antibody (ab113956) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from 293T cells at 50 µg
    Lane 2 : Whole cell lysate from 293T cells at 15 µg
    Lane 3 : Whole cell lysate from 293T cells at 5 µg

    Developed using the ECL technique

    Predicted band size : 64 kDa


    Exposure time : 3 minutes
  • ab113956, at 1 µg/ml, staining Cdc7 Kinase in 293T whole cell lysate immunoprecipitated using ab113956 at 3 µg/mg lysate (1 mg for IP; 20% of IP loaded). Detection: Chemiluminescence with an exposure time of 30 seconds.

References for Anti-Cdc7 Kinase antibody (ab113956)

ab113956 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (4-12 Bis tris)
Sample Human Cell lysate - whole cell (293T)
Specification 293T
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
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Submitted Aug 14 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"