Anti-Cdc7 Kinase antibody [DCS-341] (ab10535)

Overview

  • Product nameAnti-Cdc7 Kinase antibody [DCS-341]
    See all Cdc7 Kinase primary antibodies
  • Description
    Mouse monoclonal [DCS-341] to Cdc7 Kinase
  • Tested applicationsSuitable for: ELISA, WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human).

  • Positive control
    • A431 whole cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab10535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 0.4 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 64 kDa).
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

Target

  • FunctionSeems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cdc 7 antibody
    • CDC7 antibody
    • CDC7 cell division cycle 7 antibody
    • CDC7 cell division cycle 7 like 1 antibody
    • CDC7 L1 antibody
    • Cdc7 like 1 antibody
    • CDC7 related kinase antibody
    • CDC7-related kinase antibody
    • CDC7_HUMAN antibody
    • CDC7L 1 antibody
    • CDC7L1 antibody
    • Cell division cycle 7 homolog antibody
    • Cell division cycle 7 kinase antibody
    • Cell division cycle 7 like protein 1 antibody
    • Cell division cycle 7 related protein kinase antibody
    • Cell division cycle 7-related protein kinase antibody
    • HsCDC 7 antibody
    • HsCdc7 antibody
    • Hsk 1 antibody
    • Hsk1 antibody
    • HuCDC 7 antibody
    • huCdc7 antibody
    • MGC117361 antibody
    • MGC126237 antibody
    • MGC126238 antibody
    see all

Anti-Cdc7 Kinase antibody [DCS-341] images

  • All lanes : Anti-Cdc7 Kinase antibody [DCS-341] (ab10535) at 1/1000 dilution

    Lane 1 : U2OS Cell lysate with siRNA control (scramble siRNA) siCdc7
    Lane 2 : U2OS Cell Lysate with siRNA against Cdc7

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-Mouse monoclonal conjugated to HRP at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 64 kDa

    This image is courtesy of an Abreview submitted by Remi Buisson

    Blocking Step: 1 hour at room temperature with 5% Milk in TBS.

    Diluent: 5% Milk in TBS.

    See Abreview



  • Predicted band size : 64 kDa

    Image from Heffernan TP et al, J Biol Chem. 2007 Mar 30;282(13):9458-68. Epub 2007 Feb 2, Fig 7, DOI 10.1074/jbc.M611292200

    FLAG-Dbf4 forms a complex with endogenous Cdc7 following DNA damage. HeLa cells were transiently transfected with a FLAG-Dbf4 expression vector. 48 hours later cells were treated with 8 J/m2 UVC, 10 µm etoposide, or 2 mm HU. Cells were harvested 1 hour later and FLAG-Dbf4 immunoprecipitated with anti-FLAG-agarose (NS corresponds to immunoprecipitation with normal mouse IgG). The effect of DNA damage on Cdc7-Dbf4 complex formation was measured by Western immunoblot analysis using ab10535.

References for Anti-Cdc7 Kinase antibody [DCS-341] (ab10535)

This product has been referenced in:
  • Suzuki T  et al. Inhibition of DNA damage-induced apoptosis through Cdc7-mediated stabilization of Tob. J Biol Chem 287:40256-65 (2012). ICC/IF, Dot blot ; Human . Read more (PubMed: 23066029) »
  • Petermann E  et al. Chk1 promotes replication fork progression by controlling replication initiation. Proc Natl Acad Sci U S A 107:16090-5 (2010). WB ; Human . Read more (PubMed: 20805465) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (10 %)
Sample Human Cell lysate - whole cell (U2OS)
Specification U2OS
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Remi Buisson

Verified customer

Submitted Oct 31 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293T)
Loading amount 50 µg
Specification HEK293T
Treatment Cdc7 siRNA
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jun 01 2012

Thank you for your enquiry. As far as we are aware, cross reactivity with yeast has not yet been tested for use with ab10535. All species cross-reactivity information is stated on Abcam product datasheets, and these are updated as soon as any new in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"