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Full length protein (Human).
Our Abpromise guarantee covers the use of ab10535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|WB||Use a concentration of 0.4 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 64 kDa).|
|IP||Use at an assay dependent dilution.|
|ICC||Use at an assay dependent dilution.|
This image is courtesy of an Abreview submitted by Remi Buisson
Blocking Step: 1 hour at room temperature with 5% Milk in TBS.
Diluent: 5% Milk in TBS.
Image from Heffernan TP et al, J Biol Chem. 2007 Mar 30;282(13):9458-68. Epub 2007 Feb 2, Fig 7, DOI 10.1074/jbc.M611292200FLAG-Dbf4 forms a complex with endogenous Cdc7 following DNA damage. HeLa cells were transiently transfected with a FLAG-Dbf4 expression vector. 48 hours later cells were treated with 8 J/m2 UVC, 10 µm etoposide, or 2 mm HU. Cells were harvested 1 hour later and FLAG-Dbf4 immunoprecipitated with anti-FLAG-agarose (NS corresponds to immunoprecipitation with normal mouse IgG). The effect of DNA damage on Cdc7-Dbf4 complex formation was measured by Western immunoblot analysis using ab10535.