• Product name
  • Description
    Rabbit polyclonal to CDCA2
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 950 to the C-terminus of Human CDCA2.

    (Peptide available as ab45267.)

  • Positive control
    • This antibody gave a positive control in the following human lysates: Hela nuclear HepG2 nuclear Jurkat nuclear (data not shown)



Our Abpromise guarantee covers the use of ab45129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 113 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • Relevance
    CDCA2 is a regulator of chromosome structure during mitosis. CDCA2 forms an essential complex with protein phosphatase PP1-gamma subunit 1 (PPP1CC) and is required for the recruitment of PP1 to chromatin. Knockdown of CDCA2 by RNAi causes large-scale cell death by apoptosis, as does overexpression of a dominant-negative mutant of the protein.
  • Cellular localization
  • Database links
  • Alternative names
    • cell division cycle associated 2 antibody
    • PPP1R81 antibody
    • protein phosphatase 1 [gamma] (PPP1R3A) binding protein Repo-Man antibody
    • protein phosphatase 1, regulatory subunit 81 antibody
    • Recruits PP1 onto mitotic chromatin at anaphase protein antibody
    • Repo Man antibody
    • Repo-Man antibody
    see all

Anti-CDCA2 antibody images

  • All lanes : Anti-CDCA2 antibody (ab45129) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HepG2 nuclear extract lysate (ab14660)

    Lysates/proteins at 10 µg per lane.

    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 113 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab45129 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45129, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CDCA2 antibody (ab45129)

This product has been referenced in:
  • Qian J  et al. Cdk1 orders mitotic events through coordination of a chromosome-associated phosphatase switch. Nat Commun 6:10215 (2015). Read more (PubMed: 26674376) »
  • Wurzenberger C  et al. Sds22 and Repo-Man stabilize chromosome segregation by counteracting Aurora B on anaphase kinetochores. J Cell Biol 198:173-83 (2012). Read more (PubMed: 22801782) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (HeLa, HEK293, U2OS)
Loading amount
30 µg
HeLa, HEK293, U2OS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. Antibody Solutions

Verified customer

Submitted Oct 08 2008


Sign up