Overview

  • Product name
  • Description
    Rabbit polyclonal to CDCA3
  • Specificity
    ab69608 detects endogenous levels of total CDCA3 protein.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from the C-terminus of human CDCA3.

  • Positive control
    • Human colon carcinoma tissue, extracts from HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab69608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ELISA 1/5000.
IHC-P 1/50 - 1/100.

Target

  • Relevance
    CDCA3 (Cell division cycle associated 3) is an F-box-like protein which is required for entry into mitosis. It is associated with SKP1 as part of a SCF (SKP1-cullin-F-box) protein ligase complex, and is required for the ubiquitination and degradation of the CDK1 inhibitory tyrosine kinase WEE1 at G2/M phase.
  • Cellular localization
    Cytoplasmic. Cytosol.
  • Database links
  • Alternative names
    • C8 antibody
    • CDCA 3 antibody
    • Cell division cycle associated 3 antibody
    • Cell division cycle associated protein 3 antibody
    • Gene rich cluster C8 antibody
    • Gene rich cluster protein C8 antibody
    • GRCC 8 antibody
    • GRCC8 antibody
    • MGC2577 antibody
    • TOME 1 antibody
    • TOME1 antibody
    • Trigger of mitotic entry 1 antibody
    • Trigger of mitotic entry protein 1 antibody
    see all

Images

  • All lanes : Anti-CDCA3 antibody (ab69608) at 1/500 dilution

    Lane 1 : extracts from HepG2 cells
    Lane 2 : extracts from HepG2 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 29 kDa
    Observed band size : 29 kDa
  • Human colon carcinoma tissue stained with ab69608 at 1/50-1/100 dilution, with and without the immunising peptide.
  • ICC/IF image of ab69608 stained MCF7 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69608, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab69608 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab69608.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up