Overview

  • Product nameAnti-CDCA3 antibody
    See all CDCA3 primary antibodies
  • Description
    Rabbit polyclonal to CDCA3
  • Tested applicationsSuitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide: ELKEGAILGT GRLLKTGGRA WEQGQDHDKE NQHFPLVES, corresponding to amino acids 230-268 of human CDCA3

  • Positive control
    • HeLa whole cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab80407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
  • Application notesIP: Use at 2-5 µg/mg of lysate.

    Is unsuitable for WB. ab80407 has not performed satisfactorily when used for WB of CDCA3 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of CDCA3.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceCDCA3 (Cell division cycle associated 3) is an F-box-like protein which is required for entry into mitosis. It is associated with SKP1 as part of a SCF (SKP1-cullin-F-box) protein ligase complex, and is required for the ubiquitination and degradation of the CDK1 inhibitory tyrosine kinase WEE1 at G2/M phase.
    • Cellular localizationCytoplasmic. Cytosol.
    • Database links
    • Alternative names
      • C8 antibody
      • CDCA 3 antibody
      • Cell division cycle associated 3 antibody
      • Cell division cycle associated protein 3 antibody
      • Gene rich cluster C8 antibody
      • Gene rich cluster protein C8 antibody
      • GRCC 8 antibody
      • GRCC8 antibody
      • MGC2577 antibody
      • TOME 1 antibody
      • TOME1 antibody
      • Trigger of mitotic entry 1 antibody
      • Trigger of mitotic entry protein 1 antibody
      see all

    Anti-CDCA3 antibody images

    • Detection of human CDCA3/TOME1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. ab80407 was used for IP at 3 µg/mg lysate (Lane 1). Lane 2 represents the control IgG. For subsequent WB, 0.1 µg/ml of ab80407 was used. Detection: Chemiluminescence with an exposure time of 3 minutes.

    References for Anti-CDCA3 antibody (ab80407)

    ab80407 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab80407.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"