Overview

  • Product name
    Anti-CDCA3 antibody [EPR10226]
    See all CDCA3 primary antibodies
  • Description
    Rabbit monoclonal [EPR10226] to CDCA3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CDCA3. The exact sequence is proprietary.

  • Positive control
    • MCF7, Caco 2 and HepG2 cell lysates. MCF7 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab166902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 26 kDa.
ICC/IF 1/50 - 1/100.

Target

  • Relevance
    CDCA3 (Cell division cycle associated 3) is an F-box-like protein which is required for entry into mitosis. It is associated with SKP1 as part of a SCF (SKP1-cullin-F-box) protein ligase complex, and is required for the ubiquitination and degradation of the CDK1 inhibitory tyrosine kinase WEE1 at G2/M phase.
  • Cellular localization
    Cytoplasmic. Cytosol.
  • Database links
  • Alternative names
    • C8 antibody
    • CDCA 3 antibody
    • Cell division cycle associated 3 antibody
    • Cell division cycle associated protein 3 antibody
    • Gene rich cluster C8 antibody
    • Gene rich cluster protein C8 antibody
    • GRCC 8 antibody
    • GRCC8 antibody
    • MGC2577 antibody
    • TOME 1 antibody
    • TOME1 antibody
    • Trigger of mitotic entry 1 antibody
    • Trigger of mitotic entry protein 1 antibody
    see all

Images

  • All lanes : Anti-CDCA3 antibody [EPR10226] (ab166902) at 1/1000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : Caco 2 cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP antibody at 1/2000 dilution

    Predicted band size: 26 kDa

  • Immunofluorescent analysis of MCF7 cells labeling CDCA3 using ab166902 at 1/50 dilution.

References

This product has been referenced in:
  • Bi L  et al. A novel miR-375-HOXB3-CDCA3/DNMT3B regulatory circuitry contributes to leukemogenesis in acute myeloid leukemia. BMC Cancer 18:182 (2018). Read more (PubMed: 29439669) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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