Overview

  • Product nameAnti-CDCA5 antibody
    See all CDCA5 primary antibodies
  • Description
    Rabbit polyclonal to CDCA5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 143-192 (RRSYSRLETL GSASTSTPGR RSCFGFEGLL GAEDLSGVSP VVCSKLTEVP) of Human CDCA5 (NP_542399).

  • Positive control
    • OVCAR3 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab104780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionRegulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPAL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair.
  • Sequence similaritiesBelongs to the sororin family.
  • DomainThe KEN box is required for the association with the APC/C complex.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation, as cells enter mitosis, disrupts the interaction with PDS5A and relieves the inhibition of WAPAL by CDCA5.
    Ubiquitinated by the APC/C complex in G1, leading to its degradation.
  • Cellular localizationNucleus. Chromosome. Cytoplasm. Associates with nuclear chromatin from S phase until metaphase and is released in the cytoplasm upon nuclear envelope breakdown.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDCA5 antibody
    • CDCA5_HUMAN antibody
    • Cell division cycle associated protein 5 antibody
    • Cell division cycle-associated protein 5 antibody
    • MGC16386 antibody
    • p35 antibody
    • Sororin antibody
    see all

Anti-CDCA5 antibody images

  • Anti-CDCA5 antibody (ab104780) at 1 µg/ml + OVCAR3 cell lyate at 10 µg

    Predicted band size : 28 kDa

References for Anti-CDCA5 antibody (ab104780)

ab104780 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
Loading amount 90 µg
Specification Human osteosarcoma U2OS cells
Gel Running Conditions Reduced Denaturing (4-18% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 13 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"