Overview

  • Product name
  • Description
    Rabbit polyclonal to CDCA7
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal region of human CDCA7.

  • Positive control
    • Extracts from K562 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23166294
WB 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
ELISA 1/20000.

Target

  • Relevance
    CDCA7 is a nuclear protein without a known function, although its high homology with the transcription factor JPO2 or RAM2 suggests that it is also a transcription factor. CDCA7 is one of many target genes regulated by the c-Myc transcription factor. Overexpression of CDCA7 occurs in a significant proportion of cancers and it may play an important role in tumorigenesis. Normally it is periodically expressed in the cell cycle, peaking at the G1 to S phase transition.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • CDCA7 antibody
    • Cell division cycle associated 7 antibody
    • JPO1 antibody

Anti-CDCA7 antibody images

  • All lanes : Anti-CDCA7 antibody (ab69609) at 1/500 dilution

    Lane 1 : extracts from K562 cells
    Lane 2 : extracts from K562 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.

References for Anti-CDCA7 antibody (ab69609)

This product has been referenced in:
  • Gill RM  et al. The MYC-associated protein CDCA7 is phosphorylated by AKT to regulate MYC-dependent apoptosis and transformation. Mol Cell Biol 33:498-513 (2013). ICC/IF . Read more (PubMed: 23166294) »

See 1 Publication for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH-3T3 cells)
Permeabilization
No
Specification
NIH-3T3 cells
Blocking step
1% Donkey Serum + 1% BSA + 0.1% Triton X-100, in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 02 2015

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Cow Cell lysate - whole cell (MDBK cells)
Specification
MDBK cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Feb 16 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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