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Synthetic peptide: PLLNTQEPMEPAE, corresponding to C terminal amino acids 824-836 of Human CDCP1.
Our Abpromise guarantee covers the use of ab1377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. PubMed: 16823897|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 92 kDa).Can be blocked with Human CDCP1 peptide (ab22864).|
|IP||Use at an assay dependent dilution. PubMed: 17785447|