Overview

  • Product nameAnti-CDCP1 antibody
    See all CDCP1 primary antibodies
  • Description
    Rabbit polyclonal to CDCP1
  • Specificityab65241 detects endogenous levels of total CDCP1 protein.
  • Tested applicationsSuitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide, corresponding to internal sequence of Human CDCP1

  • Positive control
    • COLO205 cell extracts and HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab65241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).

Target

  • FunctionMay be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.
  • Tissue specificityHighly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells.
  • Sequence similaritiesContains 1 CUB domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine by kinases of the SRC family such as SRC and YES as well as by the protein kinase C gamma/PRKCG. Dephosphorylated by phosphotyrosine phosphatases. Also phosphorylated by suramin, a heparin analog. Tyrosine phosphorylated in response to dissociation of integrin alpha-6 beta-4 from laminin-5.
    N-glycosylated.
    A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). Another peptide of 80 kDa (p80) is present in cultured keratinocytes probably due to tryptic cleavage at an unidentified site on its N-terminal side. Converted to p80 by plasmin, a trypsin-like protease.
  • Cellular localizationSecreted and Cell membrane. Shedding may also lead to a soluble peptide.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9030022E12Rik antibody
    • AA409659 antibody
    • CD 318 antibody
    • CD_antigen=CD318 antibody
    • CD318 antibody
    • CD318 antigen antibody
    • CDCP 1 antibody
    • Cdcp1 antibody
    • CDCP1_HUMAN antibody
    • CUB domain containing protein 1 antibody
    • CUB domain-containing protein 1 antibody
    • E030027H19Rik antibody
    • FLJ13772 antibody
    • FLJ22375 antibody
    • FLJ22969 antibody
    • Membrane glycoprotein gp140 antibody
    • MGC31813 antibody
    • OTTHUMP00000164120 antibody
    • OTTHUMP00000209568 antibody
    • RGD1305578 antibody
    • SIMA 135 antibody
    • SIMA135 antibody
    • Subtractive immunization M plus HEp3 associated 135 kDa protein antibody
    • Subtractive immunization M plus HEp3-associated 135 kDa protein antibody
    • Transmembrane and associated with src kinases antibody
    • TRASK antibody
    see all

Anti-CDCP1 antibody images

  • ab65241 at 1/500-1/1000 dilution staining CDCP1 in HepG2 cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.
  • All lanes : Anti-CDCP1 antibody (ab65241) at 1/500 dilution

    Lane 1 : COLO205 cell extract
    Lane 2 : COLO205 cell extract with immunising peptide


    Predicted band size : 93 kDa
    Observed band size : 93 kDa

References for Anti-CDCP1 antibody (ab65241)

ab65241 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"