Recombinant Anti-CDH12 antibody [EPR14164] (ab181860)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14164] to CDH12
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CDH12 antibody [EPR14164]
See all CDH12 primary antibodies -
Description
Rabbit monoclonal [EPR14164] to CDH12 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain and fetal heart lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14164 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 130 kDa (predicted molecular weight: 88 kDa).
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IP |
1/40.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 130 kDa (predicted molecular weight: 88 kDa). |
IP
1/40. |
Target
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Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. -
Tissue specificity
Brain. -
Sequence similarities
Contains 5 cadherin domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1010 Human
- Entrez Gene: 215654 Mouse
- Entrez Gene: 301887 Rat
- Omim: 600562 Human
- SwissProt: P55289 Human
- SwissProt: Q5RJH3 Mouse
- Unigene: 113684 Human
- Unigene: 300909 Mouse
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Alternative names
- Br cadherin antibody
- BR-cadherin antibody
- Brain cadherin antibody
see all
Images
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Western blot analysis of immunoprecipitation pellet from Human fetal heart lysate immunoprecipitated using ab181860 at 1/40 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
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All lanes : Anti-CDH12 antibody [EPR14164] (ab181860) at 1/20000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 88 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab181860 has been referenced in 4 publications.
- Miao Y et al. Histone methyltransferase SUV39H2 regulates LSD1-dependent CDH1 expression and promotes epithelial mesenchymal transition of osteosarcoma. Cancer Cell Int 21:2 (2021). PubMed: 33397384
- Guo P et al. FadA promotes DNA damage and progression of Fusobacterium nucleatum-induced colorectal cancer through up-regulation of chk2. J Exp Clin Cancer Res 39:202 (2020). PubMed: 32993749
- Wang H et al. MicroRNA-495 Inhibits Gastric Cancer Cell Migration and Invasion Possibly via Targeting High Mobility Group AT-Hook 2 (HMGA2). Med Sci Monit 23:640-648 (2017). PubMed: 28159956
- Wang JF et al. CDH12 promotes the invasion of salivary adenoid cystic carcinoma. Oncol Rep 26:101-8 (2011). PubMed: 21573496