Recombinant
RabMAb

Anti-CDK1 + Cdk2 + Cdk3 + Cdk5 antibody [E53] (ab32030)

Overview

  • Product nameAnti-CDK1 + Cdk2 + Cdk3 + Cdk5 antibody [E53]
  • Description
    Rabbit monoclonal [E53] to CDK1 + Cdk2 + Cdk3 + Cdk5
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CDK1 aa 1-100.

  • Positive control
    • WB: Hela cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab32030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
IP 1/40.
ICC/IF Use at an assay dependent concentration.
  • Application notesIs unsuitable for Flow Cyt or IHC.
  • Target

    Anti-CDK1 + Cdk2 + Cdk3 + Cdk5 antibody [E53] images

    • All lanes : Anti-CDK1 + Cdk2 + Cdk3 + Cdk5 antibody [E53] (ab32030) at 1/10000 dilution

      Lane 1 : Empty vector transfected 293T (human embryonic kidney) whole cell lysates
      Lane 2 : CDK2-GFP transfected 293T (human embryonic kidney) whole cell lysates
      Lane 3 : CDK3-GFP transfected 293T (human embryonic kidney) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 34 kDa
      Observed band size : 62 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 second

      Blocking/Diluting buffer 5% NFDM/TBST

      The endogenous CDK is detected at higher exposure.

      We are not sure the bands around 35kDa. Perhaps they are degradation products.

    • Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling CDK1 + Cdk2 + Cdk3 + Cdk5 with ab32030 at 1/200 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Staining with ab32030 at 1/200 was carried out in PBS buffer for 1 hour at 22°C. DAPI was used to counterstain. ab150081, a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody was used at 1/200 dilution.
      Under these conditions, the antibody produced a strong, nuclear-enriched focal staining pattern in interphase nuclei.

      See Abreview

    • All lanes : Anti-CDK1 + Cdk2 + Cdk3 + Cdk5 antibody [E53] (ab32030) at 1/2000 dilution

      Lane 1 : Empty vector transfected 293T (human embryonic kidney) whole cell lysates
      Lane 2 : CDK1-GFP transfected 293T (human embryonic kidney) whole cell lysates
      Lane 3 : CDK5-GFP transfected 293T (human embryonic kidney) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 34 kDa


      Exposure time : 15 seconds

      Blocking/Diluting buffer 5% NFDM/TBST

      34kDa band represents endogenous CDK, and 62kDa represents CDK-GFP.

    • Anti-CDK1 + Cdk2 + Cdk3 + Cdk5 antibody [E53] (ab32030) at 1/5000 dilution + Hela cell lysate

      Predicted band size : 34 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)

    References for Anti-CDK1 + Cdk2 + Cdk3 + Cdk5 antibody [E53] (ab32030)

    ab32030 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Permeabilization Yes - 0.5% Triton X-100 in PBS
    Specification HeLa
    Fixative Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Jan 16 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"