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Our Abpromise guarantee covers the use of ab6434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 34 kDa).|
|ELISA||Use at an assay dependent dilution.|
Western blot using ab6434 at 2
Lane 1: HeLa cells
Lane 2: 293 cells
Lane 3: Xenopus egg extract
Lane 4: Xenopus cultured cells.
Cdk 1 and Cdk 2 are the two bands at the bottom of the gel (Cdk 1 is the upper of the two bands). This antibody should not be used to distinguish between Cdk1 and 2 on the same blot. This immunoblot shows that AN21 detects both Cdk 1 and Cdk 2 from Xenopus cultured cells. Xenopus Cdk 1 and 2 are easily separated by SDS-PAGE whereas the mammalian homologous are more difficult to resolve.
Julian Gannon, CRUK.
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