Overview

  • Product nameAnti-Cdk1 + Cdk2 antibody [AN21.2]
  • Description
    Mouse monoclonal [AN21.2] to Cdk1 + Cdk2
  • SpecificityRecognises both cdk1 and cdk2 - useful as a general cdk antibody since it reacts with both cdk1 and cdk2 and is very sensitive giving an intense signal.
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human cdk2

  • EpitopeRecognises C terminal epitope.
  • Positive control
    • HeLa nuclear extract

Properties

Applications

Our Abpromise guarantee covers the use of ab6434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 34 kDa).
ELISA Use at an assay dependent dilution.

Target

  • RelevanceCdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk2 is closely related to cdc2 (cdk1) which has proved useful as a marker of proliferation. Cdk1 and Cdk2 are a catalytic subunits of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • Alternative names
    • CDC2 antibody
    • Cdc2 related protein kinase antibody
    • CDC28A antibody
    • Cell division cycle 2 antibody
    • Cyclin dependent kinase 2 antibody
    • p33 protein kinase antibody
    • p34 protein kinase antibody
    see all

Anti-Cdk1 + Cdk2 antibody [AN21.2] images



  • Predicted band size : 34 kDa


    Western blot using ab6434 at 2 µg/ml.

    Lane 1: HeLa cells

    Lane 2: 293 cells

    Lane 3: Xenopus egg extract

    Lane 4: Xenopus cultured cells.

    Cdk 1 and Cdk 2 are the two bands at the bottom of the gel (Cdk 1 is the upper of the two bands).  This antibody should not be used to distinguish between Cdk1 and 2 on the same blot. This immunoblot shows that AN21 detects both Cdk 1 and Cdk 2 from Xenopus cultured cells. Xenopus Cdk 1 and 2 are easily separated by SDS-PAGE whereas the mammalian homologous are more difficult to resolve.

    Julian Gannon, CRUK.

References for Anti-Cdk1 + Cdk2 antibody [AN21.2] (ab6434)

This product has been referenced in:
  • Huang Y  et al. ATM kinase is a master switch for the Delta Np63 alpha phosphorylation/degradation in human head and neck squamous cell carcinoma cells upon DNA damage. Cell Cycle 7:2846-55 (2008). WB ; Human . Read more (PubMed: 18769144) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"