The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
IP: Use at 2.5µl/ml of lysate.
WB: 1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPlays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
Tissue specificityIsoform 2 is found in breast cancer tissues.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
FormCDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
Cdc 2 antibody
CDK 1 antibody
CELL CYCLE CONTROLLER CDC2 antibody
Cell division control protein 2 antibody
Cell division control protein 2 homolog antibody
Cell division cycle 2 G1 to S and G2 to M antibody
Cell division protein kinase 1 antibody
Cell Divsion Cycle 2 Protein antibody
Cyclin Dependent Kinase 1 antibody
Cyclin-dependent kinase 1 antibody
p34 Cdk1 antibody
p34 protein kinase antibody
Anti-CDK1 antibody [6C9] images
Western blot - Cdc2 antibody [6C9] (ab71087)
All lanes : Anti-CDK1 antibody [6C9] (ab71087) at 1/2000 dilution
Lane 1 : K562 cell lysate Lane 2 : Jurkat T cell lysate Lane 3 : C6 cell lysate Lane 4 : NIH3T3 cell lysate