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Carboxyterminal fragment starting at aa 85 of Xenopus p34cdc2 expressed in E. coli
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Our Abpromise guarantee covers the use of ab18 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use 0.5µg for 106 cells.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 34 kDa.|
|IP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|RIA||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab18 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CDK1 (green) with ab18 at 1/500. Cells were fixed with paraformaldehyde and permeabilized with 0.05% Triton X-100 in PBS for 5 minutes. Blocked with 5% goat serum as blocking agent for 1 hour at 25°C. Alexa Fluor® 488-conjugated goat anti-mouse IgG was used as the secondary antibody. DAPI (blue) was used as a nuclear counterstain.
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