Overview

  • Product name
  • Description
    Rabbit polyclonal to CDK1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length Human Cdc2 protein with His tag.

  • Positive control
    • K562, Jurkat and C6 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab71939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at a concentration of 2.5 µl/ml of lysate.
    WB: 1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
    • Tissue specificity
      Isoform 2 is found in breast cancer tissues.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Form
      CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
    • Alternative names
      • Cdc 2 antibody
      • Cdc2 antibody
      • CDC28A antibody
      • CDK 1 antibody
      • CDK1 antibody
      • CDK1_HUMAN antibody
      • CDKN1 antibody
      • CELL CYCLE CONTROLLER CDC2 antibody
      • Cell division control protein 2 antibody
      • Cell division control protein 2 homolog antibody
      • Cell division cycle 2 G1 to S and G2 to M antibody
      • Cell division protein kinase 1 antibody
      • Cell Divsion Cycle 2 Protein antibody
      • Cyclin Dependent Kinase 1 antibody
      • Cyclin-dependent kinase 1 antibody
      • DKFZp686L20222 antibody
      • MGC111195 antibody
      • p34 Cdk1 antibody
      • p34 protein kinase antibody
      • P34CDC2 antibody
      see all

    Images

    • All lanes : Anti-CDK1 antibody (ab71939) at 1/2000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Jurkat T cell lysate
      Lane 3 : C6 cell lysate


      Predicted band size : 34 kDa
      Observed band size : 30 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 35 kDa,62 kDa. We are unsure as to the identity of these extra bands.

    References

    This product has been referenced in:

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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