Overview

  • Product nameAnti-CDK1 antibody [E161]
    See all CDK1 primary antibodies
  • Description
    Rabbit monoclonal [E161] to CDK1
  • Tested applicationsWB, IHC-P, ICC, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to internal residues of Human CDK1.

  • Positive control
    • WB: HeLa cell lysate. IHC-P: Human lymphoma.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A 40 µl trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/50.
ICC 1/500.
IP 1/50.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionPlays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
    • Tissue specificityIsoform 2 is found in breast cancer tissues.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • FormCDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
    • Alternative names
      • Cdc 2 antibody
      • Cdc2 antibody
      • CDC28A antibody
      • CDK 1 antibody
      • CDK1 antibody
      • CDK1_HUMAN antibody
      • CDKN1 antibody
      • CELL CYCLE CONTROLLER CDC2 antibody
      • Cell division control protein 2 antibody
      • Cell division control protein 2 homolog antibody
      • Cell division cycle 2 G1 to S and G2 to M antibody
      • Cell division protein kinase 1 antibody
      • Cell Divsion Cycle 2 Protein antibody
      • Cyclin Dependent Kinase 1 antibody
      • Cyclin-dependent kinase 1 antibody
      • DKFZp686L20222 antibody
      • MGC111195 antibody
      • p34 Cdk1 antibody
      • p34 Protein Kinase antibody
      • P34CDC2 antibody
      see all

    Anti-CDK1 antibody [E161] images

    • Ab32384, at a 1/50 dilution, staining Cdc2 in paraffin embedded human lymphoma tissue sections by Immunohistochemistry.
    • All lanes : Anti-CDK1 antibody [E161] (ab32384) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : SKBR3 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 34 kDa

    References for Anti-CDK1 antibody [E161] (ab32384)

    This product has been referenced in:
    • Wang H  et al. Cordyceps cicadae induces G2/M cell cycle arrest in MHCC97H human hepatocellular carcinoma cells: a proteomic study. Chin Med 9:15 (2014). WB ; Human . Read more (PubMed: 24872842) »
    • Li L  et al. Cdk1 interplays with Oct4 to repress differentiation of embryonic stem cells into trophectoderm. FEBS Lett 586:4100-7 (2012). Read more (PubMed: 23108051) »

    See all 3 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (osteosarcoma cells)
    Specification osteosarcoma cells
    Treatment lane 1 control, lane 2 siRNA for CDK1, 48h
    Blocking step Milk as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 21°C
    Username

    Dr. Sonia Rocha

    Verified customer

    Submitted Sep 26 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"