Fill in the missing piece on your journey to publication Enter the draw

Overview

  • Product nameAnti-CDK1 antibody [E161]
    See all CDK1 primary antibodies
  • Description
    Rabbit monoclonal [E161] to CDK1
  • Tested applicationsWB, IHC-P, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CDK1 aa 1-100.
    Database link: P06493

  • Positive control
    • WB: HeLa cell lysate. IHC-P: Human lymphoma.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    A trial size is available to purchase for this antibody.

     

    Alternative versions available:

    Anti-CDK1 antibody (Alexa Fluor® 488) [E161] (ab193829)

    Anti-CDK1 antibody (HRP) [E161] (ab193834)

Properties

Applications

Our Abpromise guarantee covers the use of ab32384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol)

IP 1/50.
ICC/IF 1/100 - 1/500.

Target

  • FunctionPlays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
  • Tissue specificityIsoform 2 is found in breast cancer tissues.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • FormCDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
  • Alternative names
    • Cdc 2 antibody
    • Cdc2 antibody
    • CDC28A antibody
    • CDK 1 antibody
    • CDK1 antibody
    • CDK1_HUMAN antibody
    • CDKN1 antibody
    • CELL CYCLE CONTROLLER CDC2 antibody
    • Cell division control protein 2 antibody
    • Cell division control protein 2 homolog antibody
    • Cell division cycle 2 G1 to S and G2 to M antibody
    • Cell division protein kinase 1 antibody
    • Cell Divsion Cycle 2 Protein antibody
    • Cyclin Dependent Kinase 1 antibody
    • Cyclin-dependent kinase 1 antibody
    • DKFZp686L20222 antibody
    • MGC111195 antibody
    • p34 Cdk1 antibody
    • p34 protein kinase antibody
    • P34CDC2 antibody
    see all

Anti-CDK1 antibody [E161] images

  • Anti-CDK1 antibody [E161] (ab32384) at 1/5000 dilution (purified) + Raw264.7 cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size : 34 kDa
    Observed band size : 34 kDa

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-CDK1 antibody [E161] (ab32384) at 1/10000 dilution (purified)

    Lane 1 : C6 cell lysate
    Lane 2 : PC-12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size : 34 kDa
    Observed band size : 34 kDa

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-CDK1 antibody [E161] (ab32384) at 1/10000 dilution (purified)

    Lane 1 : A431 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : SK-BR-3 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size : 34 kDa
    Observed band size : 34 kDa

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunofluorescence staining of HeLa cells with purified ab32384 at a working dilution of 1/200, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab32384 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Immunohistochemical staining of paraffin embedded human ovarian carcinoma with purified ab32384 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemical staining of paraffin embedded human B cell lymphoma with purified ab32384 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • ab32384 (purified) at 1/50 immunoprecipitating CDK1 in 10 μg HEK293 (Lanes 1 and 2, observed at 34 kDa). Lane 3 - PBS. For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
  • Unpurified ab32384, at a 1/50 dilution, staining Cdc2 in paraffin embedded human lymphoma tissue sections by Immunohistochemistry.

    Ab32384, at a 1/50 dilution, staining Cdc2 in paraffin embedded human lymphoma tissue sections by Immunohistochemistry.
  • All lanes : Anti-CDK1 antibody [E161] (ab32384) at 1/1000 dilution (unpurified)

    Lane 1 : A431 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : SKBR3 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 34 kDa

References for Anti-CDK1 antibody [E161] (ab32384)

This product has been referenced in:
  • Wang H  et al. Cordyceps cicadae induces G2/M cell cycle arrest in MHCC97H human hepatocellular carcinoma cells: a proteomic study. Chin Med 9:15 (2014). WB ; Human . Read more (PubMed: 24872842) »
  • Li L  et al. Cdk1 interplays with Oct4 to repress differentiation of embryonic stem cells into trophectoderm. FEBS Lett 586:4100-7 (2012). Read more (PubMed: 23108051) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (osteosarcoma cells)
Specification osteosarcoma cells
Treatment lane 1 control, lane 2 siRNA for CDK1, 48h
Blocking step Milk as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Dr. Sonia Rocha

Verified customer

Submitted Sep 26 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"