Overview

  • Product nameAnti-CDK1 antibody [E161]
    See all CDK1 primary antibodies
  • Description
    Rabbit monoclonal [E161] to CDK1
  • Tested applicationsWB, IHC-P, ICC, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to internal residues of Human CDK1.

  • Positive control
    • WB: HeLa cell lysate. IHC-P: Human lymphoma.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

     

    Alternative versions:

    Anti-CDK1 antibody (Alexa Fluor® 488) [E161] (ab193829)

    Anti-CDK1 antibody (HRP) [E161] (ab193834)

     

Properties

Applications

Our Abpromise guarantee covers the use of ab32384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/50.
ICC 1/500.
IP 1/50.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionPlays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
    • Tissue specificityIsoform 2 is found in breast cancer tissues.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • FormCDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
    • Alternative names
      • Cdc 2 antibody
      • Cdc2 antibody
      • CDC28A antibody
      • CDK 1 antibody
      • CDK1 antibody
      • CDK1_HUMAN antibody
      • CDKN1 antibody
      • CELL CYCLE CONTROLLER CDC2 antibody
      • Cell division control protein 2 antibody
      • Cell division control protein 2 homolog antibody
      • Cell division cycle 2 G1 to S and G2 to M antibody
      • Cell division protein kinase 1 antibody
      • Cell Divsion Cycle 2 Protein antibody
      • Cyclin Dependent Kinase 1 antibody
      • Cyclin-dependent kinase 1 antibody
      • DKFZp686L20222 antibody
      • MGC111195 antibody
      • p34 Cdk1 antibody
      • p34 Protein Kinase antibody
      • P34CDC2 antibody
      see all

    Anti-CDK1 antibody [E161] images

    • Ab32384, at a 1/50 dilution, staining Cdc2 in paraffin embedded human lymphoma tissue sections by Immunohistochemistry.
    • All lanes : Anti-CDK1 antibody [E161] (ab32384) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : SKBR3 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 34 kDa

    References for Anti-CDK1 antibody [E161] (ab32384)

    This product has been referenced in:
    • Wang H  et al. Cordyceps cicadae induces G2/M cell cycle arrest in MHCC97H human hepatocellular carcinoma cells: a proteomic study. Chin Med 9:15 (2014). WB ; Human . Read more (PubMed: 24872842) »
    • Li L  et al. Cdk1 interplays with Oct4 to repress differentiation of embryonic stem cells into trophectoderm. FEBS Lett 586:4100-7 (2012). Read more (PubMed: 23108051) »

    See all 3 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (osteosarcoma cells)
    Specification osteosarcoma cells
    Treatment lane 1 control, lane 2 siRNA for CDK1, 48h
    Blocking step Milk as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 21°C
    Username

    Dr. Sonia Rocha

    Verified customer

    Submitted Sep 26 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"