Recombinant Anti-CDK1 antibody [EPR165] - BSA and Azide free (ab224269)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR165] to CDK1 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-CDK1 antibody [EPR165] - BSA and Azide free
See all CDK1 primary antibodies -
Description
Rabbit monoclonal [EPR165] to CDK1 - BSA and Azide free -
Host species
Rabbit -
Specificity
We have preliminary internal testing data to indicate this antibody doesn’t react with CDK2, CDK3 and CDK5. Please contact us for more information.
-
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HeLa, Saos-2, Namalwa, and Jurkat whole cell lysate (ab7899). IHC-P: Human tonsil tissue. IP: HeLa. Flow Cyt: Jurkat cells.
-
General notes
ab224269 is the carrier-free version of ab133327.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR165 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
- Anti-CDK1 antibody [EPR165] (ab133327)
- PE Anti-CDK1 antibody [EPR165] (ab305701)
- APC Anti-CDK1 antibody [EPR165] (ab305702)
- HRP Anti-CDK1 antibody [EPR165] (ab305703)
- Alexa Fluor® 488 Anti-CDK1 antibody [EPR165] (ab309683)
- Alexa Fluor® 647 Anti-CDK1 antibody [EPR165] (ab310046)
- Alexa Fluor® 594 Anti-CDK1 antibody [EPR165] (ab310433)
- Alexa Fluor® 555 Anti-CDK1 antibody [EPR165] (ab311960)
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
Use at an assay dependent concentration.
|
|
IP |
Use at an assay dependent concentration.
|
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 34 kDa.
|
Notes |
---|
Flow Cyt (Intra)
Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 34 kDa. |
Target
-
Function
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. -
Tissue specificity
Isoform 2 is found in breast cancer tissues. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 983 Human
- Entrez Gene: 12534 Mouse
- Omim: 116940 Human
- SwissProt: P06493 Human
- SwissProt: P11440 Mouse
- Unigene: 334562 Human
- Unigene: 281367 Mouse
-
Form
CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage. -
Alternative names
- Cdc 2 antibody
- Cdc2 antibody
- CDC28A antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling CDK1 with ab133327 at 1/500 dilution (0.6 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889, an anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml). ab150077, a Goat anti-rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution (2 μg/ml). DAPI nuclear counterstain. Confocal image showing strong nuclear and weakly cytoplasmic staining on HeLa cell line.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133327).
-
Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling CDK1 with purified ab133327 at 1/130 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor®488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133327).
-
This IHC data was generated using the same anti-CDK1 antibody clone [EPR165] in a different buffer formulation (cat# ab133327).
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling CDK1 with ab133327 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
This data was developed using ab133327, the same antibody clone in a different buffer formulation.
CDK1 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with ab133327 at 1/20 dilution (2µg) . VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2: abab133327 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab133327 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (5)
ab224269 has been referenced in 5 publications.
- Cheng N et al. Benzyl Isothiocyanate Induces Apoptosis and Inhibits Tumor Growth in Canine Mammary Carcinoma via Downregulation of the Cyclin B1/Cdk1 Pathway. Front Vet Sci 7:580530 (2020). PubMed: 33263014
- Gao K et al. HDGF-related protein-2 (HRP-2) acts as an oncogene to promote cell growth in hepatocellular carcinoma. Biochem Biophys Res Commun 458:849-55 (2015). WB ; Human . PubMed: 25689719
- Fritsche MK et al. Cisplatin fails to induce puma mediated apoptosis in mucosal melanomas. Oncotarget 6:9887-96 (2015). IHC-P ; Human . PubMed: 25831048
- Wang JF et al. The molecular mechanisms of Tanshinone IIA on the apoptosis and arrest of human esophageal carcinoma cells. Biomed Res Int 2014:582730 (2014). WB ; Human . PubMed: 24829906
- Wu JM et al. Aurora kinase inhibitors reveal mechanisms of HURP in nucleation of centrosomal and kinetochore microtubules. Proc Natl Acad Sci U S A 110:E1779-87 (2013). PubMed: 23610398