Overview

  • Product nameAnti-CDK1 antibody [POH-1]
    See all CDK1 primary antibodies
  • Description
    Mouse monoclonal [POH-1] to CDK1
  • SpecificityReacts with Cdc2
  • Tested applicationsSuitable for: ICC/IF, IP, WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human, Mink (Mustela), Monkey
    Does not react with: Rat, Saccharomyces cerevisiae, Xenopus laevis, Drosophila melanogaster, Schizosaccharomyces pombe
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab8040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 34 kDa.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionPlays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
  • Tissue specificityIsoform 2 is found in breast cancer tissues.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • FormCDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
  • Alternative names
    • Cdc 2 antibody
    • Cdc2 antibody
    • CDC28A antibody
    • CDK 1 antibody
    • CDK1 antibody
    • CDK1_HUMAN antibody
    • CDKN1 antibody
    • CELL CYCLE CONTROLLER CDC2 antibody
    • Cell division control protein 2 antibody
    • Cell division control protein 2 homolog antibody
    • Cell division cycle 2 G1 to S and G2 to M antibody
    • Cell division protein kinase 1 antibody
    • Cell Divsion Cycle 2 Protein antibody
    • Cyclin Dependent Kinase 1 antibody
    • Cyclin-dependent kinase 1 antibody
    • DKFZp686L20222 antibody
    • MGC111195 antibody
    • p34 Cdk1 antibody
    • p34 protein kinase antibody
    • P34CDC2 antibody
    see all

Anti-CDK1 antibody [POH-1] images

  • Ab8040 staining human normal skin. Staining is localised to the cytoplasm.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ICC/IF image of ab8040 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8040, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-CDK1 antibody [POH-1] (ab8040) at 1/3000 dilution + whole tissue lysate prepared from murine liver at 100 µg

    Secondary
    HRP conjugated goat anti-mouse polyclonal
    Developed using the ECL technique

    Predicted band size : 34 kDa
    Observed band size : 34 kDa


    Exposure time : 20 seconds

    Image courtesy of an anonymous Abreview.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab8040 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8040, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References for Anti-CDK1 antibody [POH-1] (ab8040)

This product has been referenced in:
  • Wang J  et al. Prognostic significance of G2/M arrest signaling pathway proteins in advanced non-small cell lung cancer patients. Oncol Lett 9:1266-1272 (2015). IHC ; Human . Read more (PubMed: 25663895) »
  • Gutierrez GJ  et al. Interplay between Cdh1 and JNK activity during the cell cycle. Nat Cell Biol 12:686-95 (2010). WB ; Human . Read more (PubMed: 20581839) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Human Cell lysate - whole cell (Glioma)
Specification Glioma
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Nov 27 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 100 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Mar 19 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"