The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use neat for 10 minutes at room temperature. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol, by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
Isoform 2 is found in breast cancer tissues.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
Cdc 2 antibody
CDK 1 antibody
CELL CYCLE CONTROLLER CDC2 antibody
Cell division control protein 2 antibody
Cell division control protein 2 homolog antibody
Cell division cycle 2 G1 to S and G2 to M antibody