Recombinant Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] (ab183550)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17499] to Cdk1 + Cdk2 (phospho T14)
- Suitable for: WB, IHC-P, IP, Dot blot
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499]
See all Cdk1 + Cdk2 primary antibodies -
Description
Rabbit monoclonal [EPR17499] to Cdk1 + Cdk2 (phospho T14) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IP, Dot blotmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa whole cell lysate treated with 2mg/ml hydroxyurea for 24 hours. IHC-P: Human colon, Human colonic adenocarcinoma, Mouse spleen and Rat spleen tissues. IP: HeLa whole cell extract.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17499 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
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IHC-P |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/40.
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Dot blot |
1/1000.
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Notes |
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WB
1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/40. |
Dot blot
1/1000. |
Target
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Relevance
Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk2 is closely related to cdc2 (cdk1) which has proved useful as a marker of proliferation. Cdk1 and Cdk2 are a catalytic subunits of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 1017 Human
- Entrez Gene: 983 Human
- SwissProt: P06493 Human
- SwissProt: P24941 Human
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Alternative names
- CDC2 antibody
- Cdc2 related protein kinase antibody
- CDC28A antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Cdk1 + Cdk2 (phospho T14) with ab183550 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Scattered nuclear and cytoplasmic staining on epithelial cells of Human colon tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling Cdk1 + Cdk2 (phospho T14) with ab183550 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and cytoplasmic staining on cancer cells of Human colonic adenocarcinoma tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Cdk1 + Cdk2 (phospho T14) with ab183550 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and cytoplasmic staining on lymphocytes of mouse spleen tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Cdk1 + Cdk2 (phospho T14) with ab183550 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and cytoplasmic staining on lymphocytes of rat spleen tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Cdk1 + Cdk2 (phospho T14) was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab183550 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab183550 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution.
Lane 1 (Input): HeLa whole cell extract 10 µg (Input).
Lane 2: ab183550 IP in HeLa whole cell extract.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab183550 in HeLa whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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Dot blot analysis of Cdk1 + Cdk2 (phospho T14) peptide (Lane 1), and non-phospho peptide (Lane 2), labeled using ab183550 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time = 3 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab183550 has been referenced in 1 publication.
- Gött H et al. ZEB1 induces N-cadherin expression in human glioblastoma and may alter patient survival. Mol Clin Oncol 17:123 (2022). PubMed: 35911664