Recombinant
RabMAb

Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] (ab183550)

Overview

  • Product name
    Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499]
    See all Cdk1 + Cdk2 primary antibodies
  • Description
    Rabbit monoclonal [EPR17499] to Cdk1 + Cdk2 (phospho T14)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IP, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Cdk1 + Cdk2 aa 1-100 (phospho T14). The exact sequence is proprietary. Also UniProt - P24941
    Database link: P06493

  • Positive control
    • WB: HeLa whole cell lysate treated with 2mg/ml hydroxyurea for 24 hours. IHC-P: Human colon, Human colonic adenocarcinoma, Mouse spleen and Rat spleen tissues. IP: HeLa whole cell extract.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab183550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/40.
Dot blot 1/1000.

Target

  • Relevance
    Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk2 is closely related to cdc2 (cdk1) which has proved useful as a marker of proliferation. Cdk1 and Cdk2 are a catalytic subunits of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle.
  • Cellular localization
    Cytoplasmic and Nuclear
  • Database links
  • Alternative names
    • CDC2 antibody
    • Cdc2 related protein kinase antibody
    • CDC28A antibody
    • Cell division cycle 2 antibody
    • Cyclin dependent kinase 2 antibody
    • p33 protein kinase antibody
    • p34 protein kinase antibody
    see all

Images

  • All lanes : Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] (ab183550) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with 2mg/ml hydroxyurea for 24 hours.
    Lane 2 : Untreated HeLa whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time = 30 seconds

     

    Sequences at the Immunogen region are identical between Cdk1 and Cdk2.

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Cdk1 + Cdk2 (phospho T14) with ab183550 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Scattered nuclear and cytoplasmic staining on epithelial cells of Human colon tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling Cdk1 + Cdk2 (phospho T14) with ab183550 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and cytoplasmic staining on cancer cells of Human colonic adenocarcinoma tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Cdk1 + Cdk2 (phospho T14) with ab183550 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and cytoplasmic staining on lymphocytes of mouse spleen tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Cdk1 + Cdk2 (phospho T14) with ab183550 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and cytoplasmic staining on lymphocytes of rat spleen tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Cdk1 + Cdk2 (phospho T14) was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab183550 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab183550 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution.

    Lane 1 (Input): HeLa whole cell extract 10 µg (Input).

    Lane 2: ab183550 IP in HeLa whole cell extract.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab183550 in HeLa whole cell extract.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Dot blot analysis of Cdk1 + Cdk2 (phospho T14) peptide (Lane 1), and non-phospho peptide (Lane 2), labeled using ab183550 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time = 3 minutes

References

ab183550 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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