Overview

  • Product name
    Anti-CDK1 (phospho T14) antibody
    See all CDK1 primary antibodies
  • Description
    Rabbit polyclonal to CDK1 (phospho T14)
  • Specificity
    Detects endogenous levels of CDC2 only when phosphorylated at threonine 14.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human Cdc2 around the phosphorylation site of threonine 14 (E-G-TP-Y-G).

  • General notes
    Stable at -20°C for 1 year

Properties

Applications

Our Abpromise guarantee covers the use of ab58509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 34 kDa).
ELISA 1/5000.
ICC/IF 1/100 - 1/500.

Target

  • Function
    Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
  • Tissue specificity
    Isoform 2 is found in breast cancer tissues.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Form
    CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
  • Alternative names
    • Cdc 2 antibody
    • Cdc2 antibody
    • CDC28A antibody
    • CDK 1 antibody
    • CDK1 antibody
    • CDK1_HUMAN antibody
    • CDKN1 antibody
    • CELL CYCLE CONTROLLER CDC2 antibody
    • Cell division control protein 2 antibody
    • Cell division control protein 2 homolog antibody
    • Cell division cycle 2 G1 to S and G2 to M antibody
    • Cell division protein kinase 1 antibody
    • Cell Divsion Cycle 2 Protein antibody
    • Cyclin Dependent Kinase 1 antibody
    • Cyclin-dependent kinase 1 antibody
    • DKFZp686L20222 antibody
    • MGC111195 antibody
    • p34 Cdk1 antibody
    • p34 protein kinase antibody
    • P34CDC2 antibody
    see all

Images



  • Predicted band size : 34 kDa

    Western blot analysis of lysates from HepG2 cells treated with Forskolin 40nM for 30 mins, using CDK1 (phospho T14) Antibody (ab58509). The lane on the right is blocked with the phospho peptide.

  • Immunofluorescence analysis of COS7 cells using ab58509 at 1/1000 dilution.

  • All lanes : Anti-CDK1 (phospho T14) antibody (ab58509) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells, treated with Forskolin (40nM, 30mins)
    Lane 2 : Extracts from HepG2 cells, treated with Forskolin (40nM, 30mins), plus immunising peptide


    Predicted band size : 34 kDa

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab58509.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up