Anti-CDK1 (phospho T161) antibody (ab47329)


  • Product nameAnti-CDK1 (phospho T161) antibody
    See all CDK1 primary antibodies
  • Description
    Rabbit polyclonal to CDK1 (phospho T161)
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthesized phosphopeptide derived from human CDC2 around the phosphorylation site of Threonine161 (V-Y-TP-H-E).

  • Positive control
    • COLO cells, K562 cells, human breast carcinoma tissue.
  • General notes

    This antibody detects CDC2 only when phosphorylated at Threonine 161.



Our Abpromise guarantee covers the use of ab47329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P Use at an assay dependent concentration.


  • FunctionPlays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
  • Tissue specificityIsoform 2 is found in breast cancer tissues.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • FormCDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
  • Alternative names
    • Cdc 2 antibody
    • Cdc2 antibody
    • CDC28A antibody
    • CDK 1 antibody
    • CDK1 antibody
    • CDK1_HUMAN antibody
    • CDKN1 antibody
    • Cell division control protein 2 antibody
    • Cell division control protein 2 homolog antibody
    • Cell division cycle 2 G1 to S and G2 to M antibody
    • Cell division protein kinase 1 antibody
    • Cell Divsion Cycle 2 Protein antibody
    • Cyclin Dependent Kinase 1 antibody
    • Cyclin-dependent kinase 1 antibody
    • DKFZp686L20222 antibody
    • MGC111195 antibody
    • p34 Cdk1 antibody
    • p34 protein kinase antibody
    • P34CDC2 antibody
    see all

Anti-CDK1 (phospho T161) antibody images

  • ab47329 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of synthesized phospho-peptide using Alkaline phosphatase Goat anti rabbit IgG (H+L) secondary antibody.
  • All lanes : Anti-CDK1 (phospho T161) antibody (ab47329) at 1/500 dilution

    Lane 1 : COLO cell extract preincubated with immunogenic phospho-peptide. with phosphopeptide at 1 µg/ml
    Lane 2 : COLO cell extract, untreated.
    Lane 3 : K362 cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
  • ICC/IF image of ab47329 stained MCF7 cells. The cells were 4% formaldehdye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47329, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CDK1 (phospho T161) antibody (ab47329)

This product has been referenced in:
  • Wang J  et al. Prognostic significance of G2/M arrest signaling pathway proteins in advanced non-small cell lung cancer patients. Oncol Lett 9:1266-1272 (2015). IHC ; Mouse . Read more (PubMed: 25663895) »

See 1 Publication for this product

Product Wall

Thank you for your email. I have just been informed by my colleague that the blocking peptide is out of stock. Therefore they will not be able to publish the datasheet hence we are unable to fulfil your request this time. I have done my best to publish...

Read More

I am very pleased to hear your customerwould like to accept our offer and test ab47329 Anti-CDK1 (phospho T161) antibody in dog.This code will give them: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an...

Read More

I am very pleased to hear your customer would like to test ab47329 in dog. As the sequence identity is 100%, I am pleased to offer them our testing discount. The code will give them 1 free primary antibody before the expiration date. To redeem this off...

Read More

Thank you for your email. I have sent request to datasheet team for publishing the datasheet of blocking peptide. They will be able to publish this on Monday next week. I will then send you the catalogue number for ordering. Many thanks for having pati...

Read More

Thank you for contacting us. Isotype controls are the primary antibodies that do not have specificity for any target. These can be used as negative control and are helpful in determining the background staining due to secondary antibodies. By compa...

Read More

Thank you for contacting us. We can provide blocking peptide for ab47329 as a special request. Could you please confirm if you are interested in buying this? We unfortunately will not be able to supply the blocking peptide for ab18. I suggest t...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells)
Specification HeLa cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.05% Triton X 100 in PBS for 5 min
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (HeLa cells)
Loading amount 20 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (8% Tris Glycine)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Andrea McCollum

Verified customer

Submitted Aug 20 2010