Overview

  • Product nameAnti-CDK1 (phospho Y15) antibody
    See all CDK1 primary antibodies
  • Description
    Rabbit polyclonal to CDK1 (phospho Y15)
  • SpecificityThis antibody is specific for Cdc2.
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthesized phosphopeptide derived from human Cdc2 around the phosphorylation site of tyrosine 15 (G-T-YP-G-V)

  • Positive control
    • HepG2 cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab47594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
ELISA 1/10000.

Target

  • FunctionPlays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
  • Tissue specificityIsoform 2 is found in breast cancer tissues.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • FormCDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
  • Alternative names
    • Cdc 2 antibody
    • Cdc2 antibody
    • CDC28A antibody
    • CDK 1 antibody
    • CDK1 antibody
    • CDK1_HUMAN antibody
    • CDKN1 antibody
    • CELL CYCLE CONTROLLER CDC2 antibody
    • Cell division control protein 2 antibody
    • Cell division control protein 2 homolog antibody
    • Cell division cycle 2 G1 to S and G2 to M antibody
    • Cell division protein kinase 1 antibody
    • Cell Divsion Cycle 2 Protein antibody
    • Cyclin Dependent Kinase 1 antibody
    • Cyclin-dependent kinase 1 antibody
    • DKFZp686L20222 antibody
    • MGC111195 antibody
    • p34 Cdk1 antibody
    • p34 Protein Kinase antibody
    • P34CDC2 antibody
    see all

Anti-CDK1 (phospho Y15) antibody images

References for Anti-CDK1 (phospho Y15) antibody (ab47594)

This product has been referenced in:
  • Caretti V  et al. WEE1 kinase inhibition enhances the radiation response of diffuse intrinsic pontine gliomas. Mol Cancer Ther 12:141-50 (2013). WB ; Mouse . Read more (PubMed: 23270927) »
  • Lyons J  et al. MC1R and cAMP signaling inhibit cdc25B activity and delay cell cycle progression in melanoma cells. Proc Natl Acad Sci U S A 110:13845-50 (2013). Read more (PubMed: 23908401) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 20 µg
Specification HeLa cells
Treatment 1 - untreated, 2- nocodazole 500 ng/ml 16 h
Gel Running Conditions Reduced Denaturing (8% Tris Glycine)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Oct 13 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells)
Specification HeLa cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.05% Triton X 100 in PBS for 5 min
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Oct 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"