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Western blot - Cdk2 antibody (ab7954)
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Immunocytochemistry - Cdk2 antibody (ab7954)
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Western blot - Cdk2 antibody (ab7954)
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Product Name
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Cdk2 antibody
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See all Cdk2 antibodies (16)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Cdk2
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Immunogen
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Synthetic peptide (Human) (C terminal).
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Reacts with
(species key)
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Hu, Ms, Rat, Chk
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Specificity
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Reacts with Cdk2
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Tested applications
(see key)
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ICC, IHC-Fr, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: Use at an assay dependent dilution (see review).
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/400. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa). Can be blocked with Cdk 2 (C-term) peptide (human).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa, HL-60 and NIH / 3T3 lysate
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Cancer >> Cell cycle >> Kinases/phosphatases >> Cdks
Chromatin and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
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Relevance
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Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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PBS with 0.1% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cdk2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Cdk2 antibody images, references, products related to ab7954 and other tools.
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Cdk2 antibody images:
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 Western blot - Cdk2 antibody (ab7954)
Predicted band size : 34 kDa
ab7954 staining Cdk2 in HeLa whole cell lysate (lane A), HL-60 whole cell lysate (lane B) and NIH / 3T3 whole cell lysate (lane C).
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Immunocytochemistry - Cdk2 antibody (ab7954)
Synaptonemal complexes (red) from human spermatocytes at the pachytene stage showing CDK2 foci (green) and the kinetochore region (blue).
This picture was kindly supplied as part of the review submitted by Maria Oliver-Bonet.
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Western blot - Cdk2 antibody (ab7954)
All lanes : Cdk2 antibody (ab7954) at 1/200 dilution
Lane 1 : Whole cell lysate prepared from rat primary cortical neurons (untreated)
Lane 2 : Whole cell lysate prepared from rat primary cortical neurons (treated with drug)
Lysates/proteins at 20 µg per lane.
Secondary
HRP conjugated goat polyclonal to rabbit Ig at 1/10000 dilution
Performed under non-reducing conditions.
Predicted band size : 34 kDa
Exposure time : 2 minutes This image is courtesy of an Abreview submitted by Dr Elena Schwartz
See Abreview
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References for Cdk2 antibody (ab7954)
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This product has been used in: (two most recent references)
Zou Y et al. Characterization of nuclear localization signal in the N terminus of CUL4B and its essential role in cyclin E degradation and cell cycle progression. J Biol Chem 284:33320-32 (2009). WB; Human. PubMed: 19801544
Miranda-Carboni GA et al. A functional link between Wnt signaling and SKP2-independent p27 turnover in mammary tumors. Genes Dev 22:3121-34 (2008). WB; Human. PubMed: 19056892
See all 5 publication references for this product.
If you publish research using ab7954 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Cdk2
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Cdk2 antibody - more information
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Customer reviews (feedback) regarding Cdk2 antibody |
Customer FAQs regarding Cdk2 antibody |
Protocols for Cdk2 antibody |
Price and availability of products related to Cdk2 antibody |
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Cdk2 antibody - related products:
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Cdk2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Cdk2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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