Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
Overview
Product name
Anti-Cdk2 antibody
See all Cdk2 products (19) ...
Description
Rabbit polyclonal to Cdk2
Specificity
Reacts with Cdk2 unphosphorylated and phosporylated forms.
Tested applications
IHC-Fr, IP, WB, ICCmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Human
Immunogen
Synthetic peptide (Human) (C terminal).
Positive control
HeLa, HL-60 and NIH / 3T3 lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
PBS with 0.1% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Kinases/phosphatases >> Cdks
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdks
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/400Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
ICC: Use at an assay dependent dilution. (See Abreview.)
Target
Function
Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
Target information above from: UniProt accessionP24941
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cdc2 related protein kinase antibody
- Cdk 2 antibody
- CDK2 antibody
see all
Database links
- Entrez Gene: 1017 Human
- Entrez Gene: 12566 Mouse
- Entrez Gene: 362817 Rat
- Omim: 116953 Human
- SwissProt: P24941 Human
- SwissProt: P97377 Mouse
- SwissProt: Q63699 Rat
- Unigene: 19192 Human
see all
Anti-Cdk2 antibody images:
Western blot - Cdk2 antibody (ab7954)
Predicted band size : 34 kDa
ab7954 staining Cdk2 in HeLa whole cell lysate (lane A), HL-60 whole cell lysate (lane B) and NIH / 3T3 whole cell lysate (lane C).
Immunocytochemistry - Cdk2 antibody (ab7954)
Synaptonemal complexes (red) from human spermatocytes at the pachytene stage showing CDK2 foci (green) and the kinetochore region (blue).
This picture was kindly supplied as part of the review submitted by Maria Oliver-Bonet.
Western blot - Cdk2 antibody (ab7954)
All lanes : Anti-Cdk2 antibody (ab7954) at 1/200 dilution
Lane 1 : Whole cell lysate prepared from rat primary cortical neurons (untreated)
Lane 2 : Whole cell lysate prepared from rat primary cortical neurons (treated with drug)
Lysates/proteins at 20 µg per lane.
Secondary
HRP conjugated goat polyclonal to rabbit Ig at 1/10000 dilution
Performed under non-reducing conditions.
Predicted band size : 34 kDa
Exposure time : 2 minutes
This image is courtesy of an Abreview submitted by Dr Elena Schwartz
References for Anti-Cdk2 antibody (ab7954)
This product has been referenced in:
- Munkley Jet al. Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells. Nucleic Acids Res 39:2671-7 (2011). WB.Read more (PubMed: 21109536) »
- Hodeify Ret al. Cdk2-dependent phosphorylation of p21 regulates the role of Cdk2 in cisplatin cytotoxicity. Am J Physiol Renal Physiol 300:F1171-9 (2011). IP.Read more (PubMed: 21325496) »
See all 10 publications for this product
Publishing research using ab7954? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"