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Human cdk2 (cyclin-dependent kinase 2) recombinant protein
Our Abpromise guarantee covers the use of ab6433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 34 kDa).|
|ELISA||Use at an assay dependent concentration.|
|Flow Cyt||1/160. Incubate for 1 hour.
Ab18414-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Western blot using ab6433 at 5
Lane 1: HeLa
Lane 2: 293
Lane 3: mouse myeloid cells.
Julian Gannon, CRUK.
ab6433 staining human MDA-MB-436 breast cancer cells by Flow Cytometery. Cells were treated with Trypsin/EDTA for 5 minutes, sample buffer prepared as 5%FCS in PBS and gating was done on live non apoptotic cells. The primary antibody diluted 1/160 and incubated with sample for 1 hour at 4°C. A FITC conjugated horse polyclonal to mouse IgG, diluted 1/200 was used as secondary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"