Overview

  • Product nameAnti-Cdk2 antibody [AN4.3]See all Cdk2 primary antibodies ...
  • Description
    Mouse monoclonal [AN4.3] to Cdk2
  • SpecificityReacts with human, mouse and xenopus cdk2. Does not recognise other cdks.
  • Tested applicationsWB, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human, Xenopus laevis
  • Immunogen

    Human cdk2 (cyclin-dependent kinase 2) recombinant protein

  • EpitopeThe epitope is at the C-terminus.
  • Positive control
    • HeLa nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab6433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 34 kDa).
ELISA Use at an assay dependent dilution.
Flow Cyt 1/160. Incubate for 1 hour.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionInvolved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cdc2 related protein kinase antibody
      • cdc2-related protein kinase antibody
      • CDC28 antibody
      • CDC2A antibody
      • Cdk 2 antibody
      • CDK1 antibody
      • CDK2 antibody
      • CDK2_HUMAN antibody
      • CDKN2 antibody
      • Cell devision kinase 2 antibody
      • Cell division kinase 2 antibody
      • Cell division protein kinase 2 antibody
      • Cyclin dependent kinase 2 antibody
      • cyclin dependent kinase 2-alpha antibody
      • Cyclin-dependent kinase 2 antibody
      • kinase Cdc2 antibody
      • MPF antibody
      • p33 protein kinase antibody
      • p33(CDK2) antibody
      see all

    Anti-Cdk2 antibody [AN4.3] images



    • Predicted band size : 34 kDa


      Western blot using ab6433 at 5µg/ml.

      Lane 1: HeLa

      Lane 2: 293

      Lane 3: mouse myeloid cells.

      Julian Gannon, CRUK.

    • ab6433 staining human MDA-MB-436 breast cancer cells by Flow Cytometery. Cells were treated with Trypsin/EDTA for 5 minutes, sample buffer prepared as 5%FCS in PBS and gating was done on live non apoptotic cells. The primary antibody diluted 1/160 and incubated with sample for 1 hour at 4°C. A FITC conjugated horse polyclonal to mouse IgG, diluted 1/200 was used as secondary.

      See Abreview

    References for Anti-Cdk2 antibody [AN4.3] (ab6433)

    This product has been referenced in:
    • Li VC  et al. Gap 1 phase length and mouse embryonic stem cell self-renewal. Proc Natl Acad Sci U S A 109:12550-5 (2012). Read more (PubMed: 22802651) »
    • Chong HZ  et al. In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell. BMC Complement Altern Med 12:35 (2012). Flow Cyt . Read more (PubMed: 22471785) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (MDA-MB-436 breast cancer cells)
    Specification MDA-MB-436 breast cancer cells
    Preparation Cell harvesting/tissue preparation method: Trypsin/EDTA for 5 minutes
    Sample buffer: 5% FCS in PBS
    Permeabilization No
    Gating Strategy live (non apoptotic cells) were selected
    Username

    Dr. Penelope Ottewell

    Verified customer

    Submitted Dec 01 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"