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Product name Anti-Cdk2 antibody [AN4.3] See all Cdk2 primary antibodies
Mouse monoclonal [AN4.3] to Cdk2
Specificity Reacts with human, mouse and xenopus cdk2. Does not recognise other cdks.
Tested applications WB, ELISA, Flow Cyt more details
Mouse, Human, Xenopus laevis
Human cdk2 (cyclin-dependent kinase 2) recombinant protein
Epitope The epitope is at the C-terminus.
Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Phosphate buffered saline
Concentration information loading...
Purity Protein A purified
Clone number AN4.3
Light chain type unknown
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 34 kDa).
Use at an assay dependent concentration.
1/160. Incubate for 1 hour.
Application notes Is unsuitable for IP.
Function Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
Sequence similarities Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
Information by UniProt
Cdc2 related protein kinase antibody
cdc2-related protein kinase antibody
Anti-Cdk2 antibody [AN4.3] images
Western blot - Cdk2 antibody [AN4.3] (ab6433)
Predicted band size : 34 kDa
Western blot using ab6433 at 5
Lane 1: HeLa
Lane 2: 293
Lane 3: mouse myeloid cells.
Julian Gannon, CRUK.
Flow Cytometry - Cdk2 antibody [AN4.3] (ab6433) This image is courtesy of an Abreview submitted by Dr Penelope Ottewell
ab6433 staining human MDA-MB-436 breast cancer cells by Flow Cytometery. Cells were treated with Trypsin/EDTA for 5 minutes, sample buffer prepared as 5%FCS in PBS and gating was done on live non apoptotic cells. The primary antibody diluted 1/160 and incubated with sample for 1 hour at 4°C. A FITC conjugated horse polyclonal to mouse IgG, diluted 1/200 was used as secondary.
References for Anti-Cdk2 antibody [AN4.3] (ab6433)
This product has been referenced in:
et al. Gap 1 phase length and mouse embryonic stem cell self-renewal. Proc Natl Acad Sci U S A 109:12550-5 (2012).
Read more (PubMed: 22802651) »
et al. In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell. BMC Complement Altern Med 12:35 (2012).
Read more (PubMed: 22471785) »
See all 4 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"