Overview

  • Product nameAnti-Cdk2 antibody [AN4.3]
    See all Cdk2 primary antibodies
  • Description
    Mouse monoclonal [AN4.3] to Cdk2
  • SpecificityReacts with human, mouse and xenopus cdk2. Does not recognise other cdks.
  • Tested applicationsWB, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human, Xenopus laevis
  • Immunogen

    Human cdk2 (cyclin-dependent kinase 2) recombinant protein

  • EpitopeThe epitope is at the C-terminus.
  • Positive control
    • HeLa nuclear extract.
  • General notesAb18414-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab6433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 34 kDa).
ELISA Use at an assay dependent dilution.
Flow Cyt 1/160. Incubate for 1 hour.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionInvolved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cdc2 related protein kinase antibody
      • cdc2-related protein kinase antibody
      • CDC28 antibody
      • CDC2A antibody
      • Cdk 2 antibody
      • CDK1 antibody
      • CDK2 antibody
      • CDK2_HUMAN antibody
      • CDKN2 antibody
      • Cell devision kinase 2 antibody
      • Cell division kinase 2 antibody
      • Cell division protein kinase 2 antibody
      • Cyclin dependent kinase 2 antibody
      • cyclin dependent kinase 2-alpha antibody
      • Cyclin-dependent kinase 2 antibody
      • kinase Cdc2 antibody
      • MPF antibody
      • p33 protein kinase antibody
      • p33(CDK2) antibody
      see all

    Anti-Cdk2 antibody [AN4.3] images



    • Predicted band size : 34 kDa


      Western blot using ab6433 at 5µg/ml.

      Lane 1: HeLa

      Lane 2: 293

      Lane 3: mouse myeloid cells.

      Julian Gannon, CRUK.

    • ab6433 staining human MDA-MB-436 breast cancer cells by Flow Cytometery. Cells were treated with Trypsin/EDTA for 5 minutes, sample buffer prepared as 5%FCS in PBS and gating was done on live non apoptotic cells. The primary antibody diluted 1/160 and incubated with sample for 1 hour at 4°C. A FITC conjugated horse polyclonal to mouse IgG, diluted 1/200 was used as secondary.

      See Abreview

    References for Anti-Cdk2 antibody [AN4.3] (ab6433)

    This product has been referenced in:
    • Li VC  et al. Gap 1 phase length and mouse embryonic stem cell self-renewal. Proc Natl Acad Sci U S A 109:12550-5 (2012). Read more (PubMed: 22802651) »
    • Chong HZ  et al. In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell. BMC Complement Altern Med 12:35 (2012). Flow Cyt . Read more (PubMed: 22471785) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (MDA-MB-436 breast cancer cells)
    Specification MDA-MB-436 breast cancer cells
    Preparation Cell harvesting/tissue preparation method: Trypsin/EDTA for 5 minutes
    Sample buffer: 5% FCS in PBS
    Permeabilization No
    Gating Strategy live (non apoptotic cells) were selected
    Username

    Dr. Penelope Ottewell

    Verified customer

    Submitted Dec 01 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"