Overview
- Product nameAnti-Cdk5 antibody [2G2]See all Cdk5 primary antibodies ...
- DescriptionMouse monoclonal [2G2] to Cdk5
- Tested applicationsELISA, WB, IP, Sandwich ELISA more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
His-tagged recombinant human CDK5 protein purified from E. coli
- Positive controlSH-SY5Y cell lysate
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.03% Proprietary Preservative
Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride -
Concentration information loading... - PurityIgG fraction
- Clonality Monoclonal
- Clone number2G2
- IsotypeIgG1
- Light chain typekappa
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab28441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: Use at an assay dependent dilution. |
| WB | WB: 1/1000 - 1/2000. Predicted molecular weight: 33 kDa. |
| IP | IP: Use a concentration of 2 µg/ml. |
| Sandwich ELISA | sELISA: Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cdk5 (ab21249). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to Cdk5 (ab21249) as Detection. |
Target
- FunctionProbably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.
- Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. - Cellular localizationCytoplasm. Cell projection > lamellipodium. Cell projection > growth cone. In axonal growth cone with extension to the peripheral lamellipodia.
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Database links
- Entrez Gene: 1020 Human
- Entrez Gene: 12568 Mouse
- Entrez Gene: 140908 Rat
- Omim: 123831 Human
- SwissProt: Q00535 Human
- SwissProt: P49615 Mouse
- SwissProt: Q03114 Rat
- Unigene: 647078 Human
- Unigene: 298798 Mouse
- Unigene: 10749 Rat
see all
Target information above from: UniProt accession
Q00535
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Cdk 5 antibodyCdk5 antibodyCDK5_HUMAN antibody
- Cell division protein kinase 5 antibodyCrk6 antibodyCyclin dependent kinase 5 antibodyCyclin-dependent kinase 5 antibodyProtein kinase CDK5 splicing antibodyPSSALRE antibodySerine threonine protein kinase PSSALRE antibodySerine/threonine-protein kinase PSSALRE antibodyTau protein kinase II catalytic subunit antibodyTPKII catalytic subunit antibody
see all
Anti-Cdk5 antibody [2G2] images
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![Sandwich ELISA - Cdk5 antibody [2G2] (ab28441)](http://a.abcam.com/ps/datasheet/images/28/ab28441/Cdk5-Primary-antibodies-ab28441-1.jpg)
Sandwich ELISA - Cdk5 antibody [2G2] (ab28441)Standard Curve for Cdk5 (Analyte: Cdk5 protein (Tagged) (ab56282)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [2G2] to Cdk5 (ab28441) at 5µg/ml and Detector Antibody Rabbit polyclonal to Cdk5 (ab21249) at 0.5µg/ml. -
![Western blot - Cdk5 antibody [2G2] (ab28441)](http://a.abcam.com/ps/datasheet/Images/28/ab28441/ab28441_1.JPG)
Western blot - Cdk5 antibody [2G2] (ab28441)All lanes : Anti-Cdk5 antibody [2G2] (ab28441) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : SK-N-SH cell lysate
Lane 3 : Mouse brain tissue
Predicted band size : 33 kDa
Protocols
References for Anti-Cdk5 antibody [2G2] (ab28441)
ab28441 has not yet been referenced specifically in any publications.
