Recombinant
RabMAb

Anti-Cdk5 antibody [EP715Y] (ab40773)

Overview

  • Product name
    Anti-Cdk5 antibody [EP715Y]
    See all Cdk5 primary antibodies
  • Description
    Rabbit monoclonal [EP715Y] to Cdk5
  • Tested applications
    Suitable for: IHC-Fr, WB, IHC-P, ICC, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
  • Immunogen

    Synthetic peptide within Human Cdk5 aa 250-350 (C terminal). The exact sequence is proprietary.

  • Positive control
    • WB: HeLa cell lysate. IHC-P: Human brain tissue. Flow Cyt: HeLa cells. IHC-Fr: Chicken midbrain tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab40773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200.
WB 1/2000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P Use at an assay dependent concentration.
ICC 1/100 - 1/250.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.

Target

  • Function
    Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Cytoplasm. Cell projection > lamellipodium. Cell projection > growth cone. In axonal growth cone with extension to the peripheral lamellipodia.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cdk 5 antibody
    • Cdk5 antibody
    • CDK5_HUMAN antibody
    • Cell division protein kinase 5 antibody
    • Crk6 antibody
    • Cyclin dependent kinase 5 antibody
    • Cyclin-dependent kinase 5 antibody
    • Protein kinase CDK5 splicing antibody
    • PSSALRE antibody
    • Serine threonine protein kinase PSSALRE antibody
    • Serine/threonine-protein kinase PSSALRE antibody
    • Tau protein kinase II catalytic subunit antibody
    • TPKII catalytic subunit antibody
    see all

Images

  • Anti-Cdk5 antibody [EP715Y] (ab40773) at 1/10000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
  • ab40773 staining Cdk5 in the SH-SY5Y cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/300). a150077 was used as the secondary antibody (1/500) and nuclei stained with DAPI.

  • Immunohistochemical analysis of frozen PFA-fixed chicken midbrain tissue cyrosection, labelling Cdk5 with ab40773 at a dilution of 1/200 incubated for 16 hours at 4°C in 0.1% PBS Triton X and 3% BSA. Blocking was with 3% BSA incubated for 1 hour at 21°C. Secondary was a goat anti-rabbit polyclonal Alexa Fluor® 488 conjugate at 1/1000.

  • Paraffin-embedded human brain tissue stained for Cdk5 with ab40773 at 1/100 dilution.

  • Flow cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Cdk5 with ab40773 (pink). Negative control Rabbit IgG (green).

  • ab40773 at 1/50 immunoprecipitating Cdk5 in HeLa whole cell lysate.

    For western blotting, the primary antibody was used at a dilution of 1/1000 and a HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

References

This product has been referenced in:
  • Xie W  et al. Phosphorylation of kinase insert domain receptor by cyclin-dependent kinase 5 at serine 229 is associated with invasive behavior and poor prognosis in prolactin pituitary adenomas. Oncotarget 7:50883-50894 (2016). IHC-P ; Human . Read more (PubMed: 27438154) »
  • Yu HP  et al. TIGAR regulates DNA damage and repair through pentosephosphate pathway and Cdk5-ATM pathway. Sci Rep 5:9853 (2015). WB . Read more (PubMed: 25928429) »

See all 7 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
Yes - 70% Ethanol O/N at 4C
Specification
Brain
Blocking step
Dako Block #X0909 as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Paraformaldehyde
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Submitted Oct 23 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Tissue (Midbrain cryosection)
Permeabilization
Yes - 0.1% PBS Triton X
Specification
Midbrain cryosection
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Paraformaldehyde
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Submitted Nov 12 2015

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10% SDS gel)
Sample
Human Cell lysate - whole cell (human iPS cell derived neurons (healthy/diseased))
Specification
human iPS cell derived neurons (healthy/diseased)
Treatment
quinolinic acid 72h (and non treated) 500nM
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Jun 17 2014

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (HeLa cell line)
Specification
HeLa cell line
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Dec 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (U2OS cells)
Specification
U2OS cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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Submitted Jul 12 2013

DISCOUNT CODE: *** Expiration date: April 1st, 2012 I am very pleased to hear you would like to accept our offer and test ab40773 in IHC-FoFr. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please subm...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293)
Loading amount
20 µg
Specification
HEK 293
Gel Running Conditions
Non-reduced Denaturing (12.5)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Aug 22 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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