Overview

  • Product nameAnti-CDK5RAP2 antibody
    See all CDK5RAP2 primary antibodies
  • Description
    Rabbit polyclonal to CDK5RAP2
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from Human CDK5RAP2 at N-terminal

  • Positive control
    • Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab111732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionPotential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation (By similarity). Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with MAPRE1, it may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends.
  • Tissue specificityWidely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Involvement in diseaseDefects in CDK5RAP2 are the cause of microcephaly primary type 3 (MCPH3) [MIM:604804]. A disorder defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits.
  • Post-translational
    modifications
    Phosphorylated in vitro by CDK5.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome. Golgi apparatus. Cytoplasm. Found in the pericentriolar region adhering to the surface of the centrosome and in the region of the centrosomal appendages. Localizes to microtubule plus ends.
  • Information by UniProt
  • Database links
  • Alternative names
    • C48 antibody
    • CDK5 activator-binding protein C48 antibody
    • CDK5 regulatory subunit associated protein 2 antibody
    • CDK5 regulatory subunit-associated protein 2 antibody
    • CDK5RAP2 antibody
    • centrosomal protein 215 kDa antibody
    • Centrosome-associated protein 215 antibody
    • CEP215 antibody
    • CK5P2_HUMAN antibody
    • MCPH3 antibody
    see all

Anti-CDK5RAP2 antibody images

  • ab111732 at 1/50 dilution staining CDK5RAP2 in paraffin-embedded Human Brain tissue by Immunohistochemistry. Left panel shows staining without immunizing peptide right panel shows staining with immunizing peptide.

References for Anti-CDK5RAP2 antibody (ab111732)

ab111732 has not yet been referenced specifically in any publications.

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